Category: 55779-48-1

On August 16, 2022, Cumberbatch, Derrick; Mori, Tetsuya; Yang, Jie; Mi, Dehui; Vinson, Paige; Weaver, C. David; Johnson, Carl Hirschie published an article.Computed Properties of 55779-48-1 The title of the article was A BRET Ca2+ sensor enables high-throughput screening in the presence of background fluorescence. And the article contained the following:

The intrinsic fluorescence of samples confounds the use of fluorescence-based sensors. This is of particular concern in high-throughput screening (HTS) applications using large chem. libraries containing intrinsically fluorescent compounds To overcome this problem, we developed a bioluminescence resonance energy transfer (BRET) Ca2+ sensor, CalfluxCTN. We demonstrated that it reliably reported changes in intracellular Ca2+ concentrations evoked by an agonist and an antagonist of the human muscarinic acetylcholine receptor M1 (hM1R) even in the presence of the fluorescent compound fluorescein, which interfered with a standard fluorescent HTS sensor (Fluo-8). In an HTS using a chem. library containing fluorescent compounds, CalfluxCTN accurately identified agonists and antagonists that were missed or miscategorized using Fluo-8. Moreover, we showed that a luciferase substrate that becomes activated only when inside cells generated long-lasting BRET signals in HTS, enabling results to be reliably compared among replicate samples for hours. Thus, the use of a self-luminescent sensor instead of a fluorescent sensor could facilitate the complete screening of chem. libraries in a high-throughput context and enable anal. of autofluorescent samples in many different applications. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Computed Properties of 55779-48-1

The Article related to bioluminescence resonance energy transfer sensor fluorescence screening, Placeholder for records without volume info and other aspects.Computed Properties of 55779-48-1

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Pyrazine | C4H4N2 – PubChem

On September 3, 2021, Burakova, Ludmila P.; Lyakhovich, Maria S.; Mineev, Konstantin S.; Petushkov, Valentin N.; Zagitova, Renata I.; Tsarkova, Aleksandra S.; Kovalchuk, Sergey I.; Yampolsky, Ilia V.; Vysotski, Eugene S.; Kaskova, Zinaida M. published an article.Computed Properties of 55779-48-1 The title of the article was Unexpected Coelenterazine Degradation Products of Beroe abyssicola Photoprotein Photoinactivation. And the article contained the following:

Ca2+-regulated photoproteins of ctenophores lose bioluminescence activity when exposed to visible light. Little is known about the chem. nature of chromophore photoinactivation. Using a total synthesis strategy, we have established the structures of 2 unusual coelenterazine products, isolated from recombinant berovin of the ctenophore Beroe abyssicola, which are Z/E isomers. We propose that during light irradiation, these derivatives are formed from 2-hydroperoxycoelenterazine via the intermediate 8a-peroxide by a mechanism reminiscent of that previously described for the auto-oxidation of green-fluorescent-protein-like chromophores. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Computed Properties of 55779-48-1

The Article related to ctenophore photoprotein inactivation light coelenterazine degradation, Placeholder for records without volume info and other aspects.Computed Properties of 55779-48-1

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Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On April 30, 2021, Giuliani, Germano; Merolla, Assunta; Paolino, Marco; Reale, Annalisa; Saletti, Mario; Blancafort, Lluis; Cappelli, Andrea; Benfenati, Fabio; Cesca, Fabrizia published an article.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one The title of the article was Stability Studies of New Caged bis-deoxy-coelenterazine Derivatives and Their Potential Use as Cellular pH Probes. And the article contained the following:

The synthesis of new bis-deoxy-coelenterazine (1) derivatives bearing ester protective groups (acetate, propionate and butyrate esters) was accomplished. Moreover, their hydrolytic stability at room temperature was evaluated in dimethylsulfoxide (DMSO) as solvent, using the NMR (NMR) spectra of the key products at different time intervals. The results showed an increasing hydrolysis rate according to longest aliphatic chain, with a half-life of 24 days of the more stable acetate derivative (4a). Furthermore, the anal. of the exptl. data revealed the greater stability of the enol tautomer in this aprotic polar solvent. This result was confirmed by theor. calculations using the d. functional theory (DFT) approach, which gave us the opportunity to propose a detailed decomposition mechanism. Addnl., the derivatives obtained were tested by bioluminescence luciferase assays to evaluate their potential use as extracellular pH-sensitive reporter substrates of luciferase. The biol. data support the idea that further structural modifications of these mols. may open promising perspectives in this field of research. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

The Article related to bis deoxy coelenterazine derivative ph probe stability, Placeholder for records without volume info and other aspects.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

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Pyrazine – Wikipedia,
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Gao, Meng; Liu, Ya-Jun published an article in 2019, the title of the article was Photoluminescence Rainbow from Coelenteramide-A Theoretical Study.Application of 55779-48-1 And the article contains the following content:

A wide variety of marine bioluminescent organisms emit light via the excited-state coelenteramide, which is produced from the coelenterazine oxidation via a series of complicated chem. reactions in protein. Photoluminescence of coelenteramide is a simple way to produce light without experiencing the intricate reactions starting from coelenterazine. To extend the color range of light emission, many coelenterazine analogs were synthesized, but mostly only produce blue and cyan fluorescence. Based on the 42 synthesized coelenterazine analogs, we theor. studied the absorption and fluorescence properties of the corresponding coelenteramide analogs. The electronic effect, steric effect, conjugated effect and solvated effect were considered. The results indicated that conjugated effect has great influence on the strength and wavelength of fluorescence and large electron transfer is beneficial to red shift. Based on the regularities, we theor. designed six coelenteramide analogs, and together with the original coelenteramide, the seven-ones emit the seven colors of rainbow via their photoluminescences. This study expands the coelenteramide fluorescence to the whole visible light region and could inspire new application. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Application of 55779-48-1

The Article related to photoluminescence rainbow coelenteramide theor study, Placeholder for records without volume info and other aspects.Application of 55779-48-1

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Pyrazine – Wikipedia,
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On October 31, 2020, Ding, Bo-Wen; Eremeeva, Elena V.; Vysotski, Eugene S.; Liu, Ya-Jun published an article.SDS of cas: 55779-48-1 The title of the article was Luminescence Activity Decreases When v-coelenterazine Replaces Coelenterazine in Calcium-Regulated Photoprotein-A Theoretical and Experimental Study. And the article contained the following:

Calcium-regulated photoproteins are found in at least five phyla of organisms. The light emitted by those photoproteins can be tuned by mutating the photoprotein and/or by modifying the substrate coelenterazine (CTZ). Thirty years ago, Shimomura observed that the luminescence activity of aequorin was dramatically reduced when the substrate CTZ was replaced by its analog v-CTZ. The latter is formed by adding a Ph ring to the π-conjugated moiety of CTZ. The decrease in luminescence activity has not been understood until now. In this paper, through combined quantum mechanics and mol. mechanics calculations as well as mol. dynamics simulations, we discovered the reason for this observation. Modification of the substrate changes the conformation of nearby aromatic residues and enhances the π-π stacking interactions between the conjugated moiety of v-CTZ and the residues, which weakens the charge transfer to form light emitter and leads to a lower luminescence activity. The microenvironments of CTZ in obelin and in aequorin are very similar, so we predicted that the luminescence activity of obelin will also dramatically decrease when CTZ is replaced by v-CTZ. This prediction has received strong evidence from currently theor. calculations and has been verified by experiments The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).SDS of cas: 55779-48-1

The Article related to coelenterazine calcium photoprotein a luminescence, General Biochemistry: Subcellular Processes and other aspects.SDS of cas: 55779-48-1

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Pyrazine – Wikipedia,
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On September 30, 2022, Inouye, Satoshi; Nakamura, Mitsuhiro; Hosoya, Takamitsu published an article.Product Details of 55779-48-1 The title of the article was Formation of Coelenteramine from 2-Peroxycoelenterazine in the Ca2+-Binding Photoprotein Aequorin. And the article contained the following:

Aequorin consists of apoprotein (apoAequorin) and (S)-2-peroxycoelenterazine (CTZ-OOH) and is considered to be a transient-state complex of an enzyme (apoAequorin) and a substrate (coelenterazine and mol. oxygen) in the enzymic reaction. The degradation process of CTZ-OOH in aequorin was characterized under various conditions of protein denaturation. By acid treatment, the major product from CTZ-OOH was coelenteramine (CTM), but not coelenteramide (CTMD), and no significant luminescence was observed The counterparts of CTM from CTZ-OOH were identified as 4-hydroxyphenylpyruvic acid (4HPPA) and 4-hydroxyphenylacetic acid (4HPAA) by liquid chromatog./electrospray ionization-time-of-flight mass spectrometry (LC/ESI-TOF-MS). In the luminescence reaction of aequorin with Ca2+, similar amounts of 4HPPA and 4HPAA were detected, indicating that CTM is formed by two pathways from CTZ-OOH through dioxetanone anion and not by hydrolysis from CTMD. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Product Details of 55779-48-1

The Article related to aequorin coelenteramine peroxycoelenterazine calcium, Biochemical Methods: Chromatographic and other aspects.Product Details of 55779-48-1

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Abe, Masahiro; Nishihara, Ryo; Ikeda, Yuma; Nakajima, Takahiro; Sato, Moritoshi; Iwasawa, Naoko; Nishiyama, Shigeru; Paulmurugan, Ramasamy; Citterio, Daniel; Kim, Sung Bae; Suzuki, Koji published an article in 2019, the title of the article was Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette.Electric Literature of 55779-48-1 And the article contains the following content:

A coelenterazine (CTZ) analog emitting near-IR (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analog, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiol. samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Electric Literature of 55779-48-1

The Article related to breast cancer fibroblast like cell nir bioluminescence imaging, bioluminescence, dyes/pigments, energy transfer, fluorescence, imaging agents, Biochemical Methods: Spectral and Related Methods and other aspects.Electric Literature of 55779-48-1

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Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On December 16, 2020, Nishihara, Ryo; Niwa, Kazuki; Tomita, Tatsunosuke; Kurita, Ryoji published an article.Category: pyrazines The title of the article was Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence. And the article contained the following:

A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an ELISA without the need for any sample pretreatments because of the high specificity of HuLumino1. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines

The Article related to coelenterazine analog serum albumin bioluminescence, Biochemical Methods: Spectral and Related Methods and other aspects.Category: pyrazines

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Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On June 2, 2020, Moutsiopoulou, Angeliki; Broyles, David; Joda, Hamdi; Dikici, Emre; Kaur, Avinash; Kaifer, Angel; Daunert, Sylvia; Deo, Sapna K. published an article.Synthetic Route of 55779-48-1 The title of the article was Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. And the article contained the following:

Although bioluminescent mol. beacons designed around resonance quenchers have shown higher signal-to-noise ratios and increased sensitivity compared with fluorescent beacon systems, bioluminescence quenching is still comparatively inefficient. A more elegant solution to inefficient quenching can be realized by designing a competitive inhibitor that is structurally very similar to the native substrate, resulting in essentially complete substrate exclusion. In this work, the authors designed a conjugated anti-interferon-γ (IFN-γ) mol. aptamer beacon (MAB) attached to a bioluminescent protein, Gaussia luciferase (GLuc), and an inhibitor mol. with a similar structure to the native substrate coelenterazine. To prove that a MAB can be more sensitive and have a better signal-to-noise ratio, a bioluminescence-based assay was developed against IFN-γ and provided an optimized, physiol. relevant detection limit of 1.0 nM. The authors believe that this inhibitor approach may provide a simple alternative strategy to standard resonance quenching in the development of high-performance mol. beacon-based biosensing systems. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1

The Article related to bioluminescence protein inhibitor aptamer beacon biosensor, Biochemical Methods: Spectral and Related Methods and other aspects.Synthetic Route of 55779-48-1

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Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On December 27, 2019, Klein, William P.; Diaz, Sebastian A.; Chiriboga, Matthew; Walper, Scott A.; Medintz, Igor L. published an article.Recommanded Product: 55779-48-1 The title of the article was Dendrimeric DNA-Based Nanoscaffolded BRET-FRET Optical Encryption Keys. And the article contained the following:

New types of unconventional encryption keys based on phys. unclonable functions are critically needed for information security. Here we prototype such a key based on the (bio)photonic interactions of multiple donor-acceptor dyes and bioluminescent enzymes as assembled on a dendrimeric DNA nanoscaffold. The ability of this optical key to generate ∼1037 unique challenge key combinations utilizing both bioluminescence resonance energy transfer and Förster resonance energy transfer-based interrogation was demonstrated, highlighting how unique codes can be generated and exchanged by different users as shared secret keys. We discuss how alterations in dye number, placement, excitation wavelength, etc. can alter code properties in unpredictable ways. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Recommanded Product: 55779-48-1

The Article related to dendrimeric dna nanoscaffold bret fret optical encryption key, Biochemical Methods: Spectral and Related Methods and other aspects.Recommanded Product: 55779-48-1

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Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem