Category: 2873-36-1

Senturk, Melih; Ercan, Fahriye; Yalcin, Serap published the artcile< The secondary metabolites produced by Lactobacillus plantarum downregulate BCL-2 and BUFFY genes on breast cancer cell line and model organism Drosophila melanogaster: molecular docking approach>, Synthetic Route of 2873-36-1, the main research area is Lactobacillus Drosophila breast cancer secondary metabolite mol docking; Apoptosis; Drosophila melanogaster; Gene expression; Lactobacillus plantarum; MCF-7; Molecular docking.

The current study was designed to evaluate the toxicity of the secondary metabolites of Lactobacillus plantarum against the human breast cancer cell line (MCF-7) and the Drosophila melanogaster. In this study, toxicity analyses of secondary metabolites of Lactobacillus plantarum were analyzed on breast cancer cells, and the Drosophila melanogaster. After application, in the MCF-7 cell line, expression levels of RRAS-2, TP53, BCL-2, APAF-1, CASP-3, FADD, CASP-7, BOK genes; in D. melanogaster; expression levels of RAS64B P53, BUFFY, DARK, DECAY, FADD, DRICE, and DEBCL genes were determined by RT-PCR. In addition, anal. of L. plantarum secondary metabolite was performed by GC-MS method and mol. binding poses of secondary metabolites and human enzymes were investigated in silico. Drosophila melanogaster being used as a model organism where some of the human genes were preserved. The IC50 value of the secondary metabolite in the MCF-7 cell line was determined to be 0.0011 mg/mL. Lethal concentration 50 (LC50) and 99 (LC99) values of secondary metabolites against fruit fly adults were 0.24 mg/mL and 0.54 mg/mL, resp. The substance detected in the secondary metabolite content and encoded as L13 (3-phenyl-1, 2, 4-benzotriazine) has been observed to have high binding affinity in the studied genes.

Cancer Chemotherapy and Pharmacology published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (DARK). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Zhang, Fengyu; Li, Bichen; Wen, Ying; Liu, Yanyang; Liu, Rong; Liu, Jing; Liu, Shao; Jiang, Yueping published the artcile< An integrated strategy for the comprehensive profiling of the chemical constituents of Aspongopus chinensis using UPLC-QTOF-MS combined with molecular networking>, Reference of 2873-36-1, the main research area is Aspongopus phytochemical UPLCQTOF MS molecular networking; Dereplication; cluster of nodes; mass spectrometry data; self-built database; targeted and untargeted strategy; traditional insect medicine.

ContextThe extracts of Aspongopus chinensis Dallas (Pentatomidae), an insect used in traditional Chinese medicine, have a complex chem. composition and possess multiple pharmacol. activities. ObjectiveThis study comprehensively characterizes the chem. constituents of A. chinensis by an integrated targeted and untargeted strategy using UPLC-QTOF-MS combined with mol. networking. Materials and methodsThe ultra-performance liquid chromatog.-tandem quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) combined with mol. networking-based dereplication was proposed to facilitate the identification of the chem. constituents of aqueous and ethanol extracts of A. chinensis. The overall strategy was designed to avoid the inefficiency and costliness of traditional techniques. The targeted compounds discovered in the A. chinensis extracts were identified by searching a self-built database, including fragment ions, precursor ion mass, and other structural information. The untargeted compounds were identified by analyzing the relationship between different categories, fragmentation pathways, mass spectrometry data, and the structure of the same cluster of nodes within the mol. network. The untargeted strategy was verified using com. standard samples under the same mass spectrometry conditions. ResultsThe proposed integrated targeted and untargeted strategy was successfully applied to the comprehensive profiling of the chem. constituents of aqueous and ethanol extracts of A. chinensis. A total of 124 compounds such as fatty acids, nucleosides, amino acids, and peptides, including 74 compounds that were reported for the first time, were identified in this study. ConclusionsThe integrated strategy using LC tandem HRMS combined with mol. networking could be popularised for the comprehensive profiling of chem. constituents of other traditional insect medicines.

Pharmaceutical Biology (Abingdon, United Kingdom) published new progress about Aspongopus chinensis. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Reference of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Li, Fangfang; Lu, Shengsheng; Xie, Xi; Fan, Sheng; Chen, Daiwei; Wu, Shaohua; He, Jian published the artcile< Antiviral properties of extracts of Streptomyces sp. SMU 03 isolated from the feces of Elephas maximus>, Reference of 2873-36-1, the main research area is Elephas Streptomyces antiviral dichloromethane extract; Anti-influenza A virus activity; Dichloromethane extracts (DCME); Elephas maximus; Membrane fusion inhibitor; Streptomyces sp. SMU03.

Actinobacteria are historically and continued to be an important source for drug discovery. The annual epidemics and periodic pandemics of humans induced by influenza A virus (IAV) prompted us to develop new effective antiviral drugs with different modes of action. An actinobacterium of Streptomyces sp. SMU 03 was identified from the feces of Elephas maximus in Yunnan Province, China. By employing an H5N1 pseudo-typed virus drug screening system, the anti-IAV effect of the dichloromethane extracts (DCME) of this bacterium was investigated. DCME showed broad and potent activities against several influenza viruses, including the H1N1 and H3N2 subtypes and influenza B virus, with IC50 values ranging from 0.37 ± 0.22 to 14.44 ± 0.79 μg/mL. Furthermore, the in vivo anti-IAV activity test of DCME showed that compared with the no-drug treated group, the survival rates, appearances, weights, lung indexes and histopathol. changes were all significantly alleviated. Based on these results, the chem. constituent study of DCME was then investigated, from which a number of antiviral compounds with various structural skeletons have been isolated and identified. Overall, these data indicated that the DCME from Streptomyces sp. SMU 03 might represent a good source for antiviral compounds that can be developed as potential antivirus remedies.

Fitoterapia published new progress about Antiviral agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Reference of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Stepien, Magdalena; Keski-Rahkonen, Pekka; Kiss, Agneta; Robinot, Nivonirina; Duarte-Salles, Talita; Murphy, Neil; Perlemuter, Gabriel; Viallon, Vivian; Tjoenneland, Anne; Rostgaard-Hansen, Agnetha Linn; Dahm, Christina C.; Overvad, Kim; Boutron-Ruault, Marie-Christine; Mancini, Francesca Romana; Mahamat-Saleh, Yahya; Aleksandrova, Krasimira; Kaaks, Rudolf; Kuehn, Tilman; Trichopoulou, Antonia; Karakatsani, Anna; Panico, Salvatore; Tumino, Rosario; Palli, Domenico; Tagliabue, Giovanna; Naccarati, Alessio; Vermeulen, Roel C. H.; Bueno-de-Mesquita, Hendrik Bastiaan; Weiderpass, Elisabete; Skeie, Guri; Ramon Quiros, Jose; Ardanaz, Eva; Mokoroa, Olatz; Sala, Nuria; Sanchez, Maria-Jose; Huerta, Jose Maria; Winkvist, Anna; Harlid, Sophia; Ohlsson, Bodil; Sjoeberg, Klas; Schmidt, Julie A.; Wareham, Nick; Khaw, Kay-Tee; Ferrari, Pietro; Rothwell, Joseph A.; Gunter, Marc; Riboli, Elio; Scalbert, Augustin; Jenab, Mazda published the artcile< Metabolic perturbations prior to hepatocellular carcinoma diagnosis: Findings from a prospective observational cohort study>, Recommanded Product: (3S,8aS)-3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione, the main research area is metabolic perturbation hepatocellular carcinoma diagnosis; hepatocellular carcinoma; prospective observational cohort; untargeted metabolomics.

Hepatocellular carcinoma (HCC) development entails changes in liver metabolism Current knowledge on metabolic perturbations in HCC is derived mostly from case-control designs, with sparse information from prospective cohorts. Our objective was to apply comprehensive metabolite profiling to detect metabolites whose serum concentrations are associated with HCC development, using biol. samples from within the prospective European Prospective Investigation into Cancer and Nutrition (EPIC) cohort (>520 000 participants), where we identified 129 HCC cases matched 1:1 to controls. We conducted high-resolution untargeted liquid chromatog.-mass spectrometry-based metabolomics on serum samples collected at recruitment prior to cancer diagnosis. Multivariable conditional logistic regression was applied controlling for dietary habits, alc. consumption, smoking, body size, hepatitis infection and liver dysfunction. Corrections for multiple comparisons were applied. Of 9206 mol. features detected, 220 discriminated HCC cases from controls. Detailed feature annotation revealed 92 metabolites associated with HCC risk, of which 14 were unambiguously identified using pure reference standards Pos. HCC-risk associations were observed for N1-acetylspermidine, isatin, p-hydroxyphenyllactic acid, tyrosine, sphingosine, L,L-cyclo(leucylprolyl), glycochenodeoxycholic acid, glycocholic acid and 7-methylguanine. Inverse risk associations were observed for retinol, dehydroepiandrosterone sulfate, glycerophosphocholine, γ-carboxyethyl hydroxychroman and creatine. Discernible differences for these metabolites were observed between cases and controls up to 10 years prior to diagnosis. Our observations highlight the diversity of metabolic perturbations involved in HCC development and replicate previous observations (metabolism of bile acids, amino acids and phospholipids) made in Asian and Scandinavian populations. These findings emphasize the role of metabolic pathways associated with steroid metabolism and immunity and specific dietary and environmental exposures in HCC development.

International Journal of Cancer published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Recommanded Product: (3S,8aS)-3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Zhou, Peng; Yan, Shan; Lu, Yuanyuan; Li, Xiao-Nian; Zhang, Mi; Li, Qin; Chen, Xia; Wang, Jianping; Zhu, Hucheng; Chen, Chunmei; Zhang, Yonghui published the artcile< Five new secondary metabolites from the fungus Phomopsis asparagi>, Application of C11H18N2O2, the main research area is analysis secondary metabolite Phomopsis spectroscopy Xray crystallog; A-glucosidase inhibition; Diketopiperazine; Diphenylcyclopentenone; Phomopsis asparagi.

Five new compounds, including a pair of diphenylcyclopentenone enantiomers (±)-phomopsisin A (1), a sesquiterpenoid 15-hydroxylithocarin A (2), a new diketopiperazine alkaloid prenylcyclotryprostatin A (3) and 7-hydroxy-cis-L(-)-3,6-dibenzyl-2,5-dioxopiperazine (6), along with five known compounds were isolated from the fungus Phomopsis asparagi. Their structures were elucidated on the basis of spectroscopic analyses (1D and 2D NMR), theor. electronic CD (ECD) calculation, modified Mosher method, and X-ray crystallog. The racemates of (±)-phomopsisin A showed inhibition on α-glucosidase with IC50 of 30.07 ± 0.75 μM (pos. control acarbose, 121 ± 2.7 μM).

Fitoterapia published new progress about Circular dichroism. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Application of C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Johnson, Shelby L.; Lu, Yu; Ma, Hang; Seeram, Navindra P.; Yuan, Tao published the artcile< Compounds from Mucuna pruriens Seeds and their Neuroprotective Effects>, SDS of cas: 2873-36-1, the main research area is Mucuna seed neuroprotective effect.

Mucuna pruriens (L.) DC., belonging to the family Fabaceae and commonly known as Mucuna or velvet bean, is a medicinal plant that in nature contains levodopa. The seeds of this medicinal plant have been used traditionally to treat brain disorders associated with Parkinson’s disease (PD) in the Indian traditional system of medicine Ayurveda. His study reports the isolation and identification of eight compounds from M. pruriens Et acetate seed extract

Chemistry of Natural Compounds published new progress about Homo sapiens. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Yang, Yin-He; Yang, Da-Song; Li, Guo-Hong; Pu, Xue-Juan; Mo, Ming-He; Zhao, Pei-Ji published the artcile< Antibacterial diketopiperazines from an endophytic fungus Bionectria sp. Y1085>, SDS of cas: 2873-36-1, the main research area is diketopiperazine isolation structure antibacterial activity Bionectria.

Two new diketopiperazines, 1 new polyprenol, together with 19 known compounds were obtained from the EtOAc extract of Bionectria sp. Y1085, an endophytic fungus isolated from the plant Huperzia serrata. Their structures were elucidated by extensive NMR and MS anal. Bionectin D (I) is a rare diketopiperazine with a single methylthio substitution at the α-carbon of cyclized amino acid residue. The antibacterial activity of compounds was assayed against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium ATCC 6539, and some metabolites exhibited evident antibacterial activity.

Journal of Antibiotics published new progress about Antibacterial agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Huang, Dan-Yu; Nong, Xu-Hua; Zhang, Yu-Qin; Xu, Wei; Sun, Long-Yu; Zhang, Tao; Chen, Guang-Ying; Han, Chang-Ri published the artcile< Two new 2,5-diketopiperazine derivatives from mangrove-derived endophytic fungus Nigrospora camelliae-sinensis S30>, Product Details of C11H18N2O2, the main research area is diketopiperazine derivative antimicrobial neuroprotective agent Nigrospora; Mangrove-derived fungus; Nigrospora camelliae-sinensis; antimicrobial activity; diketopiperazines; neuroprotective activity.

Two new 2,5-diketopiperazines derivatives, together with eight known analogs, were isolated from a culture broth of an endophytic fungus Nigrospora camelliae-sinensis S30, derived from mangrove Lumnitzera littorea. Their complete structures were determined by a detailed anal. of spectroscopic data and ECD calculations The antimicrobial activity and neuroprotective activity of these isolated compounds were also evaluated.

Natural Product Research published new progress about Antimicrobial agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Product Details of C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Salman, Mahwish; Tariq, Anam; Mustafa, Ghulam; Javed, Muhammad Rizwan; Naheed, Shazia; Qamar, Sarmad Ahmad published the artcile< Cyclo(L-Leucyl-L-Prolyl) from Lactobacillus coryniformis BCH-4 inhibits the proliferation of Aspergillus flavus: an in vitro to in silico approach>, Reference of 2873-36-1, the main research area is Lactobacillus Aspergillus proliferation Cyclo L Leucyl Prolyl antifungal bioprotectant; Antifungal potential; Aspergillus flavus; Bioprotectant; Cyclo(L-Leu-L-Pro); Lactobacillus coryniformis; Molecular docking.

Fungal spoilage led to a considerable economic loss of foodstuff which ultimately affects public health due to mycotoxins production Moreover, the consumption of com. antifungal drugs creates side effects and develops antifungal resistance. To overcome these challenges, the current work was aimed to investigate novel antifungal cyclic dipeptide (CDP) from Lactobacillus coryniformis (Loigolactobacillus coryniformis) BCH-4. CDPs have flexible, cyclic, and stable conformation. The proline-based CDPs provide addnl. structural compatibility and bio-functional values. Keeping in view, high-performance liquid chromatog. (HPLC) was performed to explore cyclo(L-Leu-L-Pro) from L. coryniformis BCH-4. The HPLC detected concentration (135 ± 7.07 mg/mL) exhibited in vitro antifungal activity of 5.66 ± 0.57 mm (inhibitory zone) against Aspergillus flavus. Based on these results, cyclo(L-Leu-L-Pro) was used as a bioprotectant for selected food samples (grapes, lemon, cashew nuts, and almonds). A significant impact of cyclo(L-Leu-L-Pro) was observed in contrast with MRS broth (control) and cell-free supernatant. In silico mol. docking anal. of this CDP was carried out against FAD glucose dehydrogenase, dihydrofolate reductase, and urate oxidase of A. flavus as target proteins. Among these proteins, FAD glucose dehydrogenase exerted strong interactions with cyclo(L-Leu-L-Pro) having S-score of – 8.21. The results evaluated that the detected CDP has strong interactions with selected proteins, causing excellent growth inhibition of A. flavus. Therefore, cyclo(L-Leu-L-Pro) could be used as a potent bioprotectant against food-borne pathogenic fungi.

Archives of Microbiology published new progress about Almond. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Reference of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Floris, Patrick; McGillicuddy, Nicola; Morrissey, Brian; Albrecht, Simone; Kaisermayer, Christian; Hawe, David; Riordan, Lelia; Lindeberg, Anna; Forestell, Sean; Bones, Jonathan published the artcile< A LC-MS/MS platform for the identification of productivity markers in industrial mammalian cell culture media>, Application In Synthesis of 2873-36-1, the main research area is mammalian cell CHO cell.

Cell culture media formulations supplemented with soy hydrolyzates for com. scale manufacturing of therapeutic proteins were characterized by high-resolution LC-MS/MS to identify specific media components that contributed to cellular bioprocess variability. Initial untargeted screening of media formulations resulted in the combined quantification of >2500 features upon anal. by reversed-phase and hydrophilic interaction liquid chromatog. Chemometric evaluation of quantified features by Principal Component Anal. and Hierarchical Clustering revealed clear correlation between media lots and the corresponding hydrolyzates implemented during formulation development stages, suggesting that the observed variation in culture performance could be influenced by components present within hydrolyzates. Through multivariate data anal., a small number of dipeptides, identified as Tyrosyl-Leucine, Phenylalanyl-Valine and LL-Cyclo(Leucylprolyl) were determined in media as potential contributors to bioprocess variability due to their statistically significant higher abundance in formulations which yielded low cellular productivity. Subsequent quantification of these features was performed through targeted LC-QQQ-MS/MS which however, revealed discrepancies between dipeptide concentration levels in media and corresponding hydrolyzates presumably due to their potential instability upon interaction with other chem. defined components such as metals or chelating agents. The nutritional impact of the aforementioned features of interest on CHO cell growth performance was assessed through spiking studies in representative shake-flask cultures which however, failed to demonstrate clear correlation between dipeptide concentrations and achieved product yields further indicating the presence of a plausible synergetic effect between the identified dipeptides and alternative media components.

Process Biochemistry (Oxford, United Kingdom) published new progress about Growth (mammalian cell). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Application In Synthesis of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem