Category: 2150-55-2

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 2150-55-2, is researched, Molecular C4H6N2O2S, about The analysis of 2-amino-2-thiazoline-4-carboxylic acid in the plasma of smokers and non-smokers, the main research direction is forensic analysis ATCA plasma human smoker GC MS; cyanide poisoning biomarker ACTA plasma human smoker GC MS; gas chromatog mass spectrometry ACTA plasma human cyanide poisoning.Application In Synthesis of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid.

ATCA (2-amino-2-thiazoline-4-carboxylic acid) is a promising marker to assess cyanide exposure because of several advantages of ATCA anal. over direct determination of cyanide and alternative cyanide biomarkers (i.e. stability in biol. matrixes, consistent recovery, and relatively small endogenous concentrations). Concentrations of ATCA in the plasma of smoking and nonsmoking human volunteers were analyzed using gas chromatog. mass spectrometry to establish the feasibility of using ATCA as a marker for cyanide exposure. The levels of ATCA in plasma of smoking volunteers, 17.2 ng/mL, were found to be significantly (p < 0.001) higher than that of nonsmoking volunteers, 11.8 ng/mL. Comparison of ATCA concentrations of smokers relative to nonsmokers in both urine and plasma yielded relatively similar results. The concentration ratio of ATCA for smokers vs. nonsmokers in plasma and urine was compared to similar literature studies of cyanide and thiocyanate, and correlations are discussed. This study supports previous evidence that ATCA can be used to determine past cyanide exposure and indicates that further studies should be pursued to validate the use of ATCA as a marker of cyanide exposure. As far as I know, this compound(2150-55-2)Application In Synthesis of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Application of 2150-55-2. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Kinetic properties of a L-cysteine desulfhydrase-deficient mutant in the enzymic formation of L-cysteine from D,L-ATC.

A mutant strain lacking L-cysteine desulfhydrase was screened after UV treatment of Pseudomonas sp. CU6. The properties of the original and mutant strains were compared on the basis of parameter values estimated from kinetic simulation of the enzymic formation of L-cysteine from D,L-2-amino-Δ2-thiazoline-4-carboxylic acid (ATC). Both strains suffered from product inhibition, but inhibition was less for the mutant strain.

As far as I know, this compound(2150-55-2)Application of 2150-55-2 can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

In general, if the atoms that make up the ring contain heteroatoms, such rings become heterocycles, and organic compounds containing heterocycles are called heterocyclic compounds. An article called Detoxication of cyanide by cystine, published in 1956, which mentions a compound: 2150-55-2, Name is 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, Molecular C4H6N2O2S, Synthetic Route of C4H6N2O2S.

cf. C.A. 46, 5633a. Chem. studies on the nature of the reaction product between cystine and cyanide support formulation of the structure as 2-imino-4-thiazolidinecarboxylic acid (I). I was inert metabolically when fed to the rat or injected. I with acid yielded a small amount of thiocyanate. I was isolated from the urine of rats given NaCN subcutaneously; 80% of the cyanide was accounted for as thiocyanate. When L-cystine-S35 was administered 1st, the compounds excreted were labeled. Radioactivity measurements showed that I came from cystine, while the thiocyanate was formed from other sources of S. The reaction with cystine constitutes an independent pathway for detoxification of cyanide. The method of Schöberl and Hamm (C.A. 43, 1014f) yielded 66% I, m. 212°, [α]D29 -2.18° (c 1, water). I with Raney Ni yielded alanine. I with Ac2O in alk. aqueous solution yielded the 3-Ac compound (II), m. 179-80°, [α]D29 -1.52° (c 1, water). I (5 g.) in EtOH saturated with dry HCl and allowed to stand 24 hrs. yielded 5 g. Et ester-HCl, m. 115-16°. The Et ester of II, m. 136°.

As far as I know, this compound(2150-55-2)Synthetic Route of C4H6N2O2S can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Huai, Lihua; Chen, Ning; Yang, Wenbo; Bai, Gang published the article 《Metabolic control analysis of L-cysteine producing strain TS1138 of Pseudomonas sp》. Keywords: model cysteine Pseudomonas metabolic control analysis.They researched the compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid( cas:2150-55-2 ).Application In Synthesis of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid. Aromatic heterocyclic compounds can be divided into two categories: single heterocyclic and fused heterocyclic. In addition, there is a lot of other information about this compound (cas:2150-55-2) here.

A kinetic model describing the biosynthesis of L-cysteine by Pseudomonas sp. TS1138 has been developed. The two enzymes catalyzing this pathway, L-cysteine synthetase (CS) and L-cysteine desulfhydrase (CD), follow Michaelis-Menten kinetics with noncompetitive inhibition of CS by L-cysteine. From measurements of intermediates and end products that were made during L-cysteine enzymic synthesis, metabolic control anal. of the pathway was carried out using the kinetic model. The elasticity coefficients and the flux control coefficients were calculated, and the anal. revealed a shift in the flux control from CS to CD during the reaction. The findings further implicate potential targets and strategies for increasing L-cysteine production; for example, the strain TS1138 could be manipulated by site-directed mutagenesis to reduce CD activity.

As far as I know, this compound(2150-55-2)Application In Synthesis of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Wang, Pu; Yin, Jiangfeng; He, Junyao; Liang, Fayong researched the compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid( cas:2150-55-2 ).Synthetic Route of C4H6N2O2S.They published the article 《Enzymatic syntheses of L-cysteine by sodium alginate/gelatin co-immobilized Pseudomonas sp. B-3》 about this compound( cas:2150-55-2 ) in Zhongguo Shipin Xuebao. Keywords: Pseudomonas cysteine sodium alginate gelatin immobilization fermentation. We’ll tell you more about this compound (cas:2150-55-2).

The immobilization of Pseudomonas sp. B-3 by sodium alginate/gelatin mixed gel and the biosynthesis of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) by immobilized cells were investigated. Suitable method for the immobilization of Pseudomonas sp. B-3 was selected by the comparison of eight immobilization methods. The influences of some key factors such as gel constitution, cells embedded and activation time on enzyme activity were optimized. Tween-60, N-carbamyl-L-cysteine amidohydrolase (L-NCC hydrolase) activator of Mn2+ and L-cysteine desulfhydrase inhibitor of hydroxylamine was added into reaction solution to improve L-cysteine productivity. Sodium alginate/gelatin co-immobilization showed both the highest enzyme activity and best gel strength. After 10 h activation for immobilized cells, the bioconversion was conducted at pH 8.0 and 42° for 10 h, 9.18 g/L-1 of L-cysteine was formed from 20 g/L-1 of DL-ATC/3H2O, with the molar conversion rate of 75.83%. An increase of 29.0% for L-cysteine production was obtained after catalyzed by immobilized cells in comparison with resting cells. After reused for four times, the relative molar conversion rate of L-cysteine remained 71.5% of the initial value. Sodium alginate/gelatin embedding method was suitable for immobilization of Pseudomonas sp. B-3. L-cysteine production was enhanced by the addition of Tween-60, Mn2+ and hydroxylamine hydrochloride in reaction solution

As far as I know, this compound(2150-55-2)Synthetic Route of C4H6N2O2S can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

HPLC of Formula: 2150-55-2. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Analysis of the cyanide metabolite 2-aminothiazoline-4-carboxylic acid in urine by high-performance liquid chromatography.

The cyanide metabolite 2-aminothiazoline-4-carboxylic acid (ATC) was converted to N-carbamylcysteine and analyzed by high-performance liquid chromatog. (HPLC) and fluorometric detection. ATC was first separated from interfering substances by passing through the cation exchanger AG 50W-X8. Interfering disulfides were converted to thiols by reduction of the eluate with thiopropyl-Sepharose 6B. The thiols were then adsorbed to the organomercurial adsorbent p-acetoxymercurianiline-Sepharose 4B while ATC was obtained in the effluent. ATC was then converted to N-carbamylcysteine by ring opening at high temperature in an alk. environment. Derivatization of N-carbamylcysteine was with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide. The imprecision (CV) of the method was 3.6% and the anal. recovery was quant. The detection limit was 0.3 μM. ATC in urine was stable for at least 3 mo when stored at -196 to +20 °C. The ATC concentration in urine was below the limit of detection in healthy nonsmokers. The utility of the method was demonstrated by the finding of up to 10.5 μM ATC concentrations in urine samples from human subjects in Mozambique living on a high dietary intake of cyanide from the cassava root. In a metabolic study rats were exposed to acetonitrile in the drinking water. After 4 wk very high ATC levels were found in the urine, representative values from two rats being 195 and 525 μM, resp.

As far as I know, this compound(2150-55-2)HPLC of Formula: 2150-55-2 can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Identification of bioconversion product from DL-ATC and optimization of reaction conditions in Pseudomonas sp. F12, published in 2014-11-20, which mentions a compound: 2150-55-2, mainly applied to Pseudomonas cysteine desulfhydrase bioconversion hydrogen sulfide, Application of 2150-55-2.

The objective if this work was to identify the bioconversion product from DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) and enhance L-cysteine yield from it. Reaction mixture was analyzed by HPLC and LC-MS; comparison among different reaction conditions of L-cysteine decomposition was performed. Contrast to standard L-cysteine, the results of HPLC and LC-MS indicated that it was L-cysteine; a small amount of hydrogen sulfide produced from degradation of L-cysteine inhibited L-cysteine desulfhydrase dramaticly in air-free condition which contributed the highest amount of L-cysteine arrived 46.2 mmol/L with a yield of 94%, in contrast to that of 31.6% under initial condition. Pseudomonas sp. F12 equipped with the ability of converting DL-ATC to L-cysteine; it was beneficial for L-cysteine accumulation in air-free condition.

As far as I know, this compound(2150-55-2)Application of 2150-55-2 can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 2150-55-2, is researched, Molecular C4H6N2O2S, about Application of 2-Aminothiazoline-4-carboxylic Acid as a Forensic Marker of Cyanide Exposure, the main research direction is forensic marker cyanide exposure aminothiazoline 4 carboxylate.Recommanded Product: 2150-55-2.

Cyanides are infamous for their highly poisonous properties. Accidental cyanide poisoning occurs frequently, but occasionally, intentional poisonings also occur. Inhalation of fumes generated by fire may also cause cyanide poisoning. There are many limitations in direct anal. of cyanide. 2-Aminothiazoline-4-carboxylic acid (ATCA), a cyanide metabolite, seems to be the only surrogate that is being used in the detection of cyanide because of its stability and its cyanide-dependent quality in biol. matrix. Unfortunately, the toxicokinetic study on diverse animal models suggests significant interspecies differences; therefore, the attempt to extrapolate animal models to human model is unsuccessful. The aim of the present study was to evaluate the use of ATCA as a forensic marker of cyanide exposure. For this purpose, postmortem materials (blood and organs) from fire victims and cyanide-poisoned persons were collected. The distribution of ATCA in organs and its thermal stability were evaluated. The variability of cyanides in purified sample and in the context of their long-term and higher temperature stability was established. The presence of ATCA was detected by using LC-MS/MS method and that of cyanide was detected spectrofluorimetrically. This is the first report on the determination of ATCA distribution in tissues of fire victims and cyanide-poisoned persons. It was found that blood and heart had the highest ATCA concentrations ATCA was observed to be thermally stable even at 90°. Even though the cyanide concentration was not elevated in purified samples, it was unstable during long-term storage and at higher temperature, as expected. The relationship between ATCA and cyanides was also observed Higher ATCA concentrations were related to increased levels of cyanide in blood and organs (less prominent). ATCA seems to be a reliable forensic marker of exposure to LDs of cyanide.

As far as I know, this compound(2150-55-2)Recommanded Product: 2150-55-2 can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Quality Control of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Cloning, expression, and identification of genes involved in the conversion of DL-2-amino-Δ2-thiazoline-4-carboxylic acid to L-cysteine via S-carbamyl-L-cysteine pathway in Pseudomonas sp. TS1138. Author is Yu, Yangsheng; Liu, Zhong; Liu, Chunqin; Li, Yang; Jin, Yongjie; Yang, Wenbo; Bai, Gang.

Two novel genes (tsB, tsC) involved in the conversion of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine through S-carbamyl-L-cysteine (L-SCC) pathway were cloned from the genomic DNA library of Pseudomonas sp. TS1138. The recombinant proteins of these two genes were expressed in Escherichia coli BL21, and their enzymic activity assays were performed in vitro. It was found that the tsB gene encoded an L-ATC hydrolase, which catalyzed the conversion of L-ATC to L-SCC, while the tsC gene encoded an L-SCC amidohydrolase, which showed the catalytic ability to convert L-SCC to L-cysteine. These results suggest that tsB and tsC play important roles in the L-SCC pathway and L-cysteine biosynthesis in Pseudomonas sp. TS1138, and that they have potential applications in the industrial production of L-cysteine.

In addition to the literature in the link below, there is a lot of literature about this compound(2-Amino-4,5-dihydrothiazole-4-carboxylic acid)Quality Control of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, illustrating the importance and wide applicability of this compound(2150-55-2).

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 2150-55-2, is researched, Molecular C4H6N2O2S, about Fed-batch fermentation kinetics of L-cysteine producing Pseudomonas strain TS1138, the main research direction is cysteine Pseudomonas.Category: pyrazines.

L-cysteine was produced by means of microbial transformation of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) in the presence of L-cysteine synthesizing enzyme from Pseudomonas sp. TS1138. Based on the exptl. data of fed-batch fermentation in 5 L fermentation reactor, the kinetic model of cell growth, enzyme production and substrate consumption were constructed. Three kinetic models were in good agreement with the exptl. results through proper anal. by software MATALAB. The kinetic models appeared to provide a reasonable description for each parameter in the fermentation

In addition to the literature in the link below, there is a lot of literature about this compound(2-Amino-4,5-dihydrothiazole-4-carboxylic acid)Category: pyrazines, illustrating the importance and wide applicability of this compound(2150-55-2).

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem