Category: pyrazines. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Optimization of fermentation conditions of microbial enzymatic synthesis of L-cysteine. Author is Kou, Guang-hui; Huai, Li-hua; Bai, Gang; Yang, Wen-bo.
Pseudomonas sp. TS1138 was used as the test strain to produce L-cysteine, and microbial enzymic synthesis of L-cysteine was studied. In the medium optimization, it was found that glucose and urea were the best carbon and nitrogen source for enzyme production, resp. Effect of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) on enzyme production was carried out, and the exptl. result showed that a initial concentration of 5 g/L DL-ATC was found to be optimal for enzyme production Effects of inoculum size, initial pH and liquid volume on enzyme production were investigated. The results indicated that the optimum inoculum size was 10%, and the optimum initial pH was 8.0. The optimum liquid volume was 40 mL culture medium in a 500 mL shaking flask.
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