Category: 2150-55-2

HPLC of Formula: 2150-55-2. The mechanism of aromatic electrophilic substitution of aromatic heterocycles is consistent with that of benzene. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Theoretical and experimental approach to hydrophilic interaction dispersive solid-phase extraction of 2-aminothiazoline-4-carboxylic acid from human post-mortem blood. Author is Giebultowicz, Joanna; Sobiech, Monika; Ruzycka, Monika; Lulinski, Piotr.

The authors proposed an innovative hydrophilic interaction dispersive solid-phase extraction (HI-d-SPE) protocol suitable for the isolation of the potential cyanide intoxication marker, 2-aminothiazoline-4-carboxylic acid (ATCA), from such complicated matrix as post-mortem blood. To create an optimal HI-d-SPE protocol, two sorbents were used: a molecularly imprinted polymer (MIP) and com. available Oasis-MCX. The latter sorbent was identified as more recovery-efficient with higher clean-up abilities in a carefully optimized process. Computational anal. was employed to provide insight into the adsorption mechanism of the two selected sorbents. The theor. results were in agreement with the experiment regarding the efficiency of the sorbent. HI-d-SPE was successfully applied to the anal. of ATCA in 20 post-mortem blood samples using LC-MS/MS. The anal. performance of the method was finally compared to prior existing methods, in turn revealing its superiority.

After consulting a lot of data, we found that this compound(2150-55-2)HPLC of Formula: 2150-55-2 can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Application of 2150-55-2. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Cysteine. Author is Nagasawa, Toru; Yamada, Hideaki.

A review with 21 references on the microbial conversion of DL-2-amino-Δ2-thiazoline-4-carboxylic acid  [2150-55-2] to L-cysteine  [52-90-4] and cystine  [56-89-3]; the enzymic synthesis of L-cysteine from β-chloro-L-alanine  [2731-73-9] and Na2S by pyridoxal phosphate-dependent enzymes; and the synthesis of D-cysteine  [921-01-7] by β-chloro-D-alanine dehydrochlorinase  [78990-65-5].

After consulting a lot of data, we found that this compound(2150-55-2)Application of 2150-55-2 can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Ninhydrin-positive components derived from cystine during the cyanide-nitroprusside test》. Authors are Hambraeus, Leif; Reio, Lembitu.The article about the compound:2-Amino-4,5-dihydrothiazole-4-carboxylic acidcas:2150-55-2,SMILESS:O=C(C1N=C(N)SC1)O).Synthetic Route of C4H6N2O2S. Through the article, more information about this compound (cas:2150-55-2) is conveyed.

NaCN used in the cyanide-nitroprusside test of Brand et. al to determine cystine (I) in urine (CA 24, 3555) could be effectively replaced by NaBH4 which yielded about the same color intensity as when NaCN was used. However, the color produced by NaBH4 faded very quickly. Studies were made to obtain some qual. and quant. data regarding the ninhydrin-pos. components during the reaction between NaCN, I, and Na nitroprusside by means of an automatic amino acid analyzer. When freshly prepared com. NaCN was used, 2 peaks were obtained (one of I and cysteine). With the addition of NaCN and I, no addnl. peak was found, but a new peak appeared which corresponded to 4-carboxythiazolidinon-2-imide (II) formed due to the cleavage of I. With pure I, peaks of II and another peak of 2-aminothiazoline-4-carboxylic acid appeared.

After consulting a lot of data, we found that this compound(2150-55-2)Synthetic Route of C4H6N2O2S can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid(SMILESS: O=C(C1N=C(N)SC1)O,cas:2150-55-2) is researched.Safety of 4-Aminopyrimidine. The article 《Seizures and selective CA-1 hippocampal lesions induced by an excitotoxic cyanide metabolite, 2-iminothiazolidine-4-carboxylic acid》 in relation to this compound, is published in Neurotoxicology. Let’s take a look at the latest research on this compound (cas:2150-55-2).

Excitatory amino acid (EAA)-like and excitotoxic properties of the secondary metabolite of cyanide, 2-iminothiazolidine-4-carboxylic acid (2-ICA), were evaluated because of its possible role in cyanide-induced neurotoxicity. Intracerebroventricular (i.c.v.) injections of 2-ICA in mice produced wild-running seizures that were qual. and quant. similar to seizures observed with glutamate. 2-ICA, kainate, and proline seizures were prevented by both the NMDA and non-NMDA antagonists, MK-801 and CNQX, resp. In contrast, NMDA-induced seizures were prevented by MK-801, but not CNQX. When infused i.c.v. in rats over a 7-day period, 2-ICA produced extensive and selective loss of CA-1 pyramidal neurons of the hippocampus. In hippocampal slices preloaded with D-[3H]aspartate, 2-ICA superfusion did not evoke release nor significantly augment potassium-stimulated release of the radiolabeled transmitter. These findings indicate 2-ICA has excitotoxic properties and its role in cyanide neurotoxicity deserves further study.

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Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Kinetic properties of a L-cysteine desulfhydrase-deficient mutant in the enzymic formation of L-cysteine from D,L-ATC.HPLC of Formula: 2150-55-2.

A mutant strain lacking L-cysteine desulfhydrase was screened after UV treatment of Pseudomonas sp. CU6. The properties of the original and mutant strains were compared on the basis of parameter values estimated from kinetic simulation of the enzymic formation of L-cysteine from D,L-2-amino-Δ2-thiazoline-4-carboxylic acid (ATC). Both strains suffered from product inhibition, but inhibition was less for the mutant strain.

After consulting a lot of data, we found that this compound(2150-55-2)HPLC of Formula: 2150-55-2 can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Screening of strains for microbial transformation from DL-ATC to L-cysteine.Quality Control of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid.

Microorganisms for bioconversion from DL-2-amino-2,3-dihydrothiazole-4-carboxylic acid (DL-ATC) to L-cysteine were isolated from 20 soil samples with DL-ATC as sole N source for isolated plate. Five bacterial strains, GJx5, GJx7, TJ4, TJ6, and TJ7, were screened. Strain GJx7 was the most effective, with the productivity 68.3 μg/mL of L-cysteine. GJx7 was genetically stable; the relative activity for the 8th generation was 95.4%. The optimum conditions for enzyme production in strain GJx7 were as follows: the optimal pH for medium was 7.0, and the optimal loading amount was 50 mL for 250 mL shake flask. The optimal C source and N source were maltose and yeast extract, resp. The productivity of L-cysteine was 320.2 μg/mL under optimum conditions.

After consulting a lot of data, we found that this compound(2150-55-2)Quality Control of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Enzymatic production of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid by Pseudomonas thiazolinophilum: optimal conditions for the enzyme formation and enzymatic reaction, published in 1978-12-31, which mentions a compound: 2150-55-2, mainly applied to Pseudomonas enzyme cysteine, Recommanded Product: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid.

Cultivation of P. thiazolinophilum AJ 3854 for the production of the enzyme which could form L-cysteine [52-90-4] from DL-2-amino-Δ2-thiazoline-4-carboxylic acid (I) [2150-55-2] and the reaction conditions of this enzyme were investigated. This enzyme was inducible, intracellular, and growth-associated A marked inactivation of enzyme was observed, especially in the growing phase, but could be prevented by 1∼10 mM Mn2+ or by I as inducer at the mid-logarithmic phase. Enzymic degradation of L-cysteine (or L-cystine [56-89-3]) formed from I could be prevented by the addition of hydroxylamine or semicarbazide. Thus, L-cysteine and L-cystine were quant. produced from I. Optimal pH and temperature of enzymic reaction were 8.2 and 42° (2 h), resp. A sigmoidal reaction curve was observed when intact cells were used as enzyme source, but sonic treatment of cells made the curve linear.

After consulting a lot of data, we found that this compound(2150-55-2)Recommanded Product: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid(SMILESS: O=C(C1N=C(N)SC1)O,cas:2150-55-2) is researched.Recommanded Product: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid. The article 《Effects of anoxic conditions on the enzymic conversion of DL-2-aminothiazoline-4-carboxylic acid to L-cystine》 in relation to this compound, is published in Acta Biotechnologica. Let’s take a look at the latest research on this compound (cas:2150-55-2).

The effects of anoxic conditions on product inhibition and the stability of L-2-aminothiazoline-4-carboxylic acid (L-ATC) hydrolase were investigated in the conversion of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) to L-cystine (I) using the cell-free extract enzyme of Pseudomonas sp. in the presence of hydroxylamine. At L-cysteine (II) equivalent levels (I:II = 1:2), I inhibited the L-ATC hydrolase reaction to a greater extent than II. In air, the product occurred predominantly as I (94.9%), whereas in a N2 atm. the product occurred as a mixture of II (39.3%) and I (40.7%). Less product inhibition took place in N. The activity of L-ATC hydrolase was almost fully lost after 20 h of incubation by shaking at 30° in air, but considerable activity remained under the anoxic conditions of N2. A kinetic anal. of the reactions confirmed that reduced product inhibition and enhanced enzyme stability in N2 result in a more efficient enzyme reaction. The inactivation rate constant (k1) was estimated to be 0.11/h in N2 and 0.22/h in air, indicating that the stability of L-ATC hydrolase in N2 was greater than in air. The values of the Kp1 and Kp2 constants related to product inhibition were 43.36 mM and 30.48 mM for II and I, resp., where higher values were an indication of less product inhibition. The value of the rate constant (k2) for the oxidation of II to I was 0.09/h in N2 and 1.01/h in air, suggesting that the II oxidation to I proceeds faster in air than in N2.

After consulting a lot of data, we found that this compound(2150-55-2)Safety of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid can be used in many types of reactions. And in most cases, this compound has more advantages.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Ninhydrin-positive components derived from cystine during the cyanide-nitroprusside test》. Authors are Hambraeus, Leif; Reio, Lembitu.The article about the compound:2-Amino-4,5-dihydrothiazole-4-carboxylic acidcas:2150-55-2,SMILESS:O=C(C1N=C(N)SC1)O).Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid. Through the article, more information about this compound (cas:2150-55-2) is conveyed.

NaCN used in the cyanide-nitroprusside test of Brand et. al to determine cystine (I) in urine (CA 24, 3555) could be effectively replaced by NaBH4 which yielded about the same color intensity as when NaCN was used. However, the color produced by NaBH4 faded very quickly. Studies were made to obtain some qual. and quant. data regarding the ninhydrin-pos. components during the reaction between NaCN, I, and Na nitroprusside by means of an automatic amino acid analyzer. When freshly prepared com. NaCN was used, 2 peaks were obtained (one of I and cysteine). With the addition of NaCN and I, no addnl. peak was found, but a new peak appeared which corresponded to 4-carboxythiazolidinon-2-imide (II) formed due to the cleavage of I. With pure I, peaks of II and another peak of 2-aminothiazoline-4-carboxylic acid appeared.

Although many compounds look similar to this compound(2150-55-2)Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, numerous studies have shown that this compound(SMILES:O=C(C1N=C(N)SC1)O), has unique advantages. If you want to know more about similar compounds, you can read my other articles.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about One-step elimination of L-cysteine desulfhydrase from crude enzyme extracts of Pseudomonas sp. TS1138 using an immunomagnetic affinity matrix improves the enzymatic production of L-cysteine.

In this study, a high efficiency immunomagnetic affinity matrix was developed to eliminate L-cysteine desulfhydrase (CD), which decomposes L-cysteine, in crude enzyme extracts from Pseudomonas sp. TS1138. After cloning and expression in Escherichia coli, recombinant CD was purified to raise polyclonal antibodies from mice. The anti-CD antibody was cross-linked to staphylococcal protein A-magnetic cellulose microspheres (MCMS) with di-Me pimelimidate (DMP). The natural CD was eliminated from the crude enzyme extracts by treatment with the cross-linked antibody-protein A-MCMS, resulting in a high level of L-cysteine production The conversion rate of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine increased significantly from 61.9 to 96.2%. The cross-linked antibody-protein A-MCMS showed its durability after repetitive use, maintaining a constant binding capacity for CD during five cycles. This study may lead to a convenient and cost-efficient method to produce L-cysteine by enzymic conversions.

Although many compounds look similar to this compound(2150-55-2)Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, numerous studies have shown that this compound(SMILES:O=C(C1N=C(N)SC1)O), has unique advantages. If you want to know more about similar compounds, you can read my other articles.

Reference:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem