The preparation of 2-amino-5-pyrazinecarboxylic acid and its derivatives was written by Felder, E.;Pitre, D.;Grabitz, E. B.. And the article was included in Helvetica Chimica Acta in 1964.Synthetic Route of C5H6N4O This article mentions the following:
2-Acetamidoquinoxazoline was treated with KMnO4 in small portions over 1 hr. at 90-5° to give 60% mono-K salt (I) of 2-acetamidopyrazine-5,6-dicarboxylic acid, m. 293° (decomposition). I (1 g.) refluxed 0.5 hr. in 35 ml. H2O with 5 ml. N HCl gave 0.7 g. 2-aminopyrazine-5,6-dicarboxylic acid, m. 262° (decomposition), which, heated in H2O 12 min. at 170-5°, gave 77% 2-aminopyrazine-5-carboxylic acid (II), m. 282-3° (decomposition); 70% Me ester (MeOH-HCl) m. 230-1°; Et ester m. 172-3°; amide m. 266-7°. II was treated in H2SO4 with NaNO2 at 5° and the mixture heated 5 min. at 80° to give 2-hydroxypyrazine-5-carboxylic acid, m. 300°. In the experiment, the researchers used many compounds, for example, 5-Aminopyrazine-2-carboxamide (cas: 89323-09-1Synthetic Route of C5H6N4O).
5-Aminopyrazine-2-carboxamide (cas: 89323-09-1) belongs to pyrazine derivatives. Pyrazine derivatives have antitumor, antibiotic, anticonvulsant, antituberculous and diuretic effects as well as kinase, enzymatic and potent tubulin and FtsZ polymerization inhibitory activities. Pyrazine heterocycles and their benzo derivatives possess many interesting properties, including chemical reactivity profiles, and have diverse applications in total synthesis, medicine, chemical biology, materials, dyes, and imaging.Synthetic Route of C5H6N4O