Liquid chromatography-mass spectrometry method for determination of tetramethylpyrazine and its metabolite in dog plasma was written by Wang, Peng;Jin, Xin;Qi, Meiling;Fang, Lin. And the article was included in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences in 2004.Product Details of 75907-74-3 This article mentions the following:
A liquid chromatog.-mass spectrometry method is described for the determination of tetramethylpyrazine (TMP) and its active metabolite, 2-hydroxymethyl-3,5,6-trimethylpyrazine (HTMP) in dog plasma. This method involves a plasma clean-up step using protein precipitation procedure followed by LC separation and pos. electrospray ionization mass spectrometry detection (ESI-MS). Chromatog. separation of the analytes was achieved on a C18 column using a mobile phase of methanol, water and acetic acid (50:50:0.6, volume/volume/v) at a flow rate of 1.0 mL/min. Selected ion monitoring (SIM) mode was used for analyte quantitation at m/z 137.2 for TMP, m/z 153.2 for HTMP and m/z 195.2 for caffeine. The linearity was obtained over the concentration ranges of 20-6000 ng/mL for TMP and 20-4000 ng/mL for HTMP and the lower limit of quantitation was 20 ng/mL for both analytes. For each level of QC samples, both inter- and intraday precisions (R.S.D.) were ≤7.4% for TMP and ≤6.0% for HTMP, and accuracy (R.E.) was ±6.0% for TMP and ≤3.5% for HTMP. The proposed LC-MS method was successfully applied to the pharmacokinetic studies of a TMP formulation preparation after oral administration to beagle dogs. In the experiment, the researchers used many compounds, for example, (3,5,6-Trimethylpyrazin-2-yl)methanol (cas: 75907-74-3Product Details of 75907-74-3).
(3,5,6-Trimethylpyrazin-2-yl)methanol (cas: 75907-74-3) belongs to pyrazine derivatives. Pyrazines are volatile compounds that are used in the cosmetic, food, flavor, and fragrance industries. Pyrazine and a variety of alkylpyrazines are flavor and aroma compounds found in baked and roasted goods. Tetramethylpyrazine (also known as ligustrazine) is reported to scavenge superoxide anion and decrease nitric oxide production in human Granulocytes.Product Details of 75907-74-3