On February 15, 2020, Hosseinnia, Maryam; Khalifeh, Khosrow; Jafarian, Vahab published an article.Synthetic Route of 55779-48-1 The title of the article was Polarity change of a representative helix in coelenterazin-binding cavity of mnemiopsin 2: Functional and structural consequences. And the article contained the following:
To evaluate the effect of a pos. charged residue at the end of the fourth helix on the function and stability of the folded state of mnemiopsin 2, Lys89 was replaced with Ala (K89A mutant). Its structural and functional features were investigated and compared with Wild type (WT) protein using a combination of theor. and exptl. procedures. The tertiary structures of WT and mutant photoproteins were made with MODELLER program, and were used for designing of mutation as well as explaining the results of exptl. measurements. Activity measurements showed that the initial intensity of light emission is reduced, but decay time is not changed upon mutation. K89A mutant exhibited more sensitivity to calcium ion than WT protein did. As compared to the pH 9 of WT, the optimum pH for the mutant increased to 9.25. According to the CD measurements at far UV region, the total amount of helical structure is not changed upon mutation; however, the helicity of chromophores is reduced in K89A mutant, demonstrating that the secondary structural elements in WT protein are more rigid. Heat-induced denaturation data showed that the stability of the WT is greater than mutant photoprotein by 2 kcal/mol. It appears that the higher value of the dipole momentum at the axis of fourth helix in WT photoprotein leads to strengthening of intra-mol. “head-to-tail” vs. “side-by-side” anti-parallel interaction between the third and fourth helixes. It was concluded that dipole-dipole interaction between the helixes are important factors in packaging of the substrate in the core structure of the photoprotein. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1
The Article related to mnemiopsin 2 coelenterazin bioluminescence thermostability dipole, General Biochemistry: Proteins and Their Constituents and other aspects.Synthetic Route of 55779-48-1