On November 30, 2020, Krasitskaya, V. V.; Bashmakova, E. E.; Kudryavtsev, A. N.; Vorobjeva, M. A.; Shatunova, E. A.; Frank, L. A. published an article.Category: pyrazines The title of the article was The Hybrid Protein ZZ-OL as an Analytical Tool for Biotechnology Research. And the article contained the following:
Abstract: The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the Ig-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Escherichia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Ca2+-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the Ig-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines
The Article related to staphylococcus escherichia hybrid protein zz ol biotechnol research, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Category: pyrazines