Eremeeva, Elena V.; Jiang, Tianyu; Malikova, Natalia P.; Li, Minyong; Vysotski, Eugene S. published an article in 2020, the title of the article was Bioluminescent properties of semi-synthetic obelin and aequorin activated by coelenterazine analogue with modifications of C-2, C-6, and C-8 substituents.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one And the article contains the following content:
Investigated the specific bioluminescence activity, light emission spectra, stopped-flow kinetics and sensitivity to calcium of the semi-synthetic aequorins and obelins activated by novel coelenterazine analogs and the recently reported coelenterazine derivatives Several semi-synthetic photoproteins activated by the studied coelenterazine analogs displayed sufficient bioluminescence activities accompanied by various changes in the spectral and kinetic properties as well as in calcium sensitivity. The poor activity of certain semi-synthetic photoproteins might be attributed to instability of some coelenterazine analogs in solution and low efficiency of 2-hydroperoxy adduct formation. In most cases, semi-synthetic obelins and aequorins displayed different properties upon being activated by the same coelenterazine analog. The results indicated that the OH-group at the C-6 Ph ring of coelenterazine was important for the photoprotein bioluminescence and that the hydrogen-bond network around the substituent in position 6 of the imidazopyrazinone core was the reason of different bioluminescence activities of aequorin and obelin with certain coelenterazine analogs. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one
The Article related to benzyl imidazopyrazinone preparation sar obelin aequorin bioluminescence, aequorin, analogues, coelenterazine, obelin, photoprotein, Heterocyclic Compounds (More Than One Hetero Atom): Pyrazines and Quinoxalines (Including Piperazines) and other aspects.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one