On October 31, 2020, Peltomaa, Riikka; Fikacek, Sabrina; Benito-Pena, Elena; Barderas, Rodrigo; Head, Trajen; Deo, Sapna; Daunert, Sylvia; Moreno-Bondi, Maria C. published an article.Category: pyrazines The title of the article was Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein. And the article contained the following:
The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G-coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL-1 (corresponding to 420μg kg-1 in food samples) and an IC50 value of 11.0 ng mL-1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the anal. of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines
The Article related to zearalenone recombinant peptidomimetic gaussia luciferase fusion protein bioluminescent detection, bioluminescence, food safety, gaussia luciferase, mycotoxin, zearalenone and other aspects.Category: pyrazines