So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Wang, Pu; Yin, Jiangfeng; He, Junyao; Liang, Fayong researched the compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid( cas:2150-55-2 ).Synthetic Route of C4H6N2O2S.They published the article 《Enzymatic syntheses of L-cysteine by sodium alginate/gelatin co-immobilized Pseudomonas sp. B-3》 about this compound( cas:2150-55-2 ) in Zhongguo Shipin Xuebao. Keywords: Pseudomonas cysteine sodium alginate gelatin immobilization fermentation. We’ll tell you more about this compound (cas:2150-55-2).
The immobilization of Pseudomonas sp. B-3 by sodium alginate/gelatin mixed gel and the biosynthesis of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) by immobilized cells were investigated. Suitable method for the immobilization of Pseudomonas sp. B-3 was selected by the comparison of eight immobilization methods. The influences of some key factors such as gel constitution, cells embedded and activation time on enzyme activity were optimized. Tween-60, N-carbamyl-L-cysteine amidohydrolase (L-NCC hydrolase) activator of Mn2+ and L-cysteine desulfhydrase inhibitor of hydroxylamine was added into reaction solution to improve L-cysteine productivity. Sodium alginate/gelatin co-immobilization showed both the highest enzyme activity and best gel strength. After 10 h activation for immobilized cells, the bioconversion was conducted at pH 8.0 and 42° for 10 h, 9.18 g/L-1 of L-cysteine was formed from 20 g/L-1 of DL-ATC/3H2O, with the molar conversion rate of 75.83%. An increase of 29.0% for L-cysteine production was obtained after catalyzed by immobilized cells in comparison with resting cells. After reused for four times, the relative molar conversion rate of L-cysteine remained 71.5% of the initial value. Sodium alginate/gelatin embedding method was suitable for immobilization of Pseudomonas sp. B-3. L-cysteine production was enhanced by the addition of Tween-60, Mn2+ and hydroxylamine hydrochloride in reaction solution
As far as I know, this compound(2150-55-2)Synthetic Route of C4H6N2O2S can be applied in many ways, which is helpful for the development of experiments. Therefore many people are doing relevant researches.