Epoxy compounds usually have stronger nucleophilic ability, because the alkyl group on the oxygen atom makes the bond angle smaller, which makes the lone pair of electrons react more dissimilarly with the electron-deficient system. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Bioconversion of D,L-ATC to L-cysteine using whole cells.Computed Properties of C4H6N2O2S.
In the conversion of D,L-2-amino-Δ2-thiazoline-4-carboxylic acid (D,L-ATC) to L-cysteine using Pseudomonas species CU6, the effects of surfactants on whole cells and the stabilities of cell-free enzyme solution and continuous reactor packed with immobilized whole cells were investigated. The enzymic reaction was minimal by whole cells without the addition of surfactants, whereas it was well carried out with SDS or Triton X-100 and was comparable to the reaction using a cell-free enzyme solution The enzyme activity of the cell-free solution decreased 50% after 7 h of storage at 30°, but not under anaerobic conditions with sparging nitrogen gas. On the other hand, there were no effects of nitrogen gas in a continuous reactor using immobilized whole cells. Hydroxylamine, an inhibitor of L-cysteine desulfhydrase, lowered the enzyme stability.
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