Most of the natural products isolated at present are heterocyclic compounds, so heterocyclic compounds occupy an important position in the research of organic chemistry. A compound: 2150-55-2, is researched, SMILESS is O=C(C1N=C(N)SC1)O, Molecular C4H6N2O2SJournal, Weishengwuxue Tongbao called Study on L-cystine conversion technology by Pseudomonas putida TS1138, Author is Liu, Chunqin; Yu, Yangsheng; Bai, Gang; Yang, Wenbo; Chen, Ning; Huai, Lihua, the main research direction is Pseudomonas cystine cysteine.Quality Control of 2-Amino-4,5-dihydrothiazole-4-carboxylic acid.
A technol. of L-cystine production was studied in this paper, which included microbial enzymic conversion of DL-2-amino-Δ2-thiazoline-4- carboxylic acid (DL-ATC) to L-cysteine, subsequent oxidization of L-cysteine to L-cystine and its purification The cells of Pseudomonas putida TS1138 could be repetitively used as the enzyme sources to convert the substrate DL-ATC to L-cysteine. After being oxidized by 2% dimethy-sulfoxide (DMSO), L-cystine could be harvested and further purified by the pos. ion-exchange resin 001*7. High Performance Liquid Chromatog. (HPLC) identified the purified L-cystine as having a total recovery of 78.55% and purity of 99.12%. This study demonstrated an efficient and convenient method for L- cystine production, which overcame the instability of enzymes, troublesome procedures and high cost of enzyme immobilization as contrasted to the traditional method. All in all, it provides a new approach for industrial production of L- cystine as well as L-cysteine.
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