In the chemical reaction process, reaction time, type of solvent, can easily affect the result of the reaction, thereby determining the yield and properties of the reaction product. An updated downstream synthesis route of 113305-94-5 as follows. Quality Control of 5-Aminopyrazine-2-carbonitrile
The 5-aminopyrazine-2-carbonitrile (commercially available, 1.0 g, 8.32 mmol) was dissolved in tetrahydrofuran then succinic anhydride (1.04 g, 10.4 mmol) was added, the mixture was stirred at room temperature for 2 h and at reflux overnight.. Then 1.0 equivalent of succinic anhydride was added and the solution was re fluxed for 4 days. The reaction was stopped and the solution was partitioned between ethyl acetate and water. The aqueous layer was separated, acidified and extracted with ethyl acetate (3x). The combined organic layer was dried on magnesium sulfate and concentrated under vaccum. The residue was purified by flash chromatography eluting with cyclohexane-ethyl acetate (1/3) with acid acetic (1%) to give 4-[(5-cyanopyrazin-2-yl)amino]-4-oxo-butanoic acid Al (1.30 g, 71%). M.p.= 201-202 C, 1H NMR (400 MHz, DMSO-d6) ? 12.09 (bs, 1H), 11.40 (s, 1H), 9.41(s, 1H), 8.96 (s, 1H), 2.73 (t, 2H), 2.55 (t, 2H) ppm. LC-MS (Method B): RT 0.96, 219 (M-H+)
According to the analysis of related databases, 113305-94-5, the application of this compound in the production field has become more and more popular.
Reference:
Patent; SYNGENTA PARTICIPATIONS AG; JUNG, Pierre Joseph Marcel; LEIPNER, Joerg; LACHIA, Mathilde Denise; DE MESMAEKER, Alain; WO2013/37753; (2013); A1;,
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem