Determination of tetramethylpyrazine and its active metabolite in rat plasma by HPLC was written by Yuan, Fang;Chen, Zhuojia;Chen, Jie. And the article was included in Zhongguo Xiandai Yingyong Yaoxue in 2012.Application of 75907-74-3 This article mentions the following:
An HPLC method for the determination of concentration of tetramethylpyrazine (TMP) and its active metabolite, 2-hydroxymethyl-3,5,6-trimethylpyrazine (HTMP) in rat plasma was established, and applied this method to the pharmacokinetic study of TMP. 2-Methylpyrazine was used as internal standard The alkalified serum samples were extracted with a chloroform-1-chloro-butane (3:1) induced liquid-liquid extraction The target analytes were quant. determined by HPLC. Hypersil BDS C18 column (4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of 20 mmol · L-1 potassium dihydrogen phosphate buffer (pH 5.6)-methanol (72:28). The flow rate was 1.0 mL · min-1, 15 μL sample was injected and detected by the ultra-violet detector at 285 nm. The linearity was obtained over the concentration ranges of 0.03125-50 μg · mL-1 for TMP (γ=0.9995) and 0.03125-5 μg · mL-1 for HTMP (γ=0.9998). The lower limit of quantitation (LLOQ) was 0.03125 μg · mL-1 for TMP and HTMP. The inter- and intra-batch precisions (RSD%) were less than 9% for both analyses. The accuracies and extracted recoveries were 86.2%-93.0% for TMP and 73.8%-95.1% for HTMP. The relative recoveries were 96.9%-117.7% for TMP and 97.5%-104.9% for HTMP. The method has good selectivity, acceptable accuracy, precision and sensitivity. It can be applied to pharmacokinetic study of TMP in rats. In the experiment, the researchers used many compounds, for example, (3,5,6-Trimethylpyrazin-2-yl)methanol (cas: 75907-74-3Application of 75907-74-3).
(3,5,6-Trimethylpyrazin-2-yl)methanol (cas: 75907-74-3) belongs to pyrazine derivatives. Pyrazine is an N-heterocyclic moiety, and it can be easily prepared from ethylenediamine and 1,2-diketone, α-hydroxyketone, α-methyl ketone. A number of pyrazine-based derivatives were used as dyes or fluorescent probes.Application of 75907-74-3