New amiloride analog as hapten to raise anti-amiloride antibodies was written by Kleyman, Thomas R.;Rajagopalan, Raghavan;Cragoe, Edward J. Jr.;Erlanger, Bernard F.;Al-Awqati, Qais. And the article was included in American Journal of Physiology in 1986.Name: Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate This article mentions the following:
A new amiloride analog amiloride-caproic acid, was synthesized, coupled to albumin, and used as a hapten to raise anti-amiloride antibodies in rabbits. The antibodies were affinity purified with an amiloride affinity column and characterized. Binding studies using [3H]benzamil showed a dissociation constant of 0.8 nM. Amiloride and amiloride-caproate inhibited [3H]benzamil binding; ε-guanidinocaproic acid showed no inhibition. Anti-amiloride antibodies reversed the inhibition by amiloride of Na transport across the toad urinary bladder. Anti-amiloride antibodies and an amiloride affinity column should provide useful tools for the characterization of the epithelial Na channel. In the experiment, the researchers used many compounds, for example, Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate (cas: 1458-01-1Name: Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate).
Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate (cas: 1458-01-1) belongs to pyrazine derivatives. Pyrazine is an N-heterocyclic moiety, and it can be easily prepared from ethylenediamine and 1,2-diketone, α-hydroxyketone, α-methyl ketone. Pyrazines are chemical compounds (technically called “methoxypyrazines”) found in grape skin and stems that are responsible for many “green” flavors in wine. Levels of pyrazines are dependent on viticultural practices, climate, and grape variety.Name: Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate