Determination and identification of amiloride in human urine by high-performance liquid chromatography and gas chromatography-mass spectrometry was written by Bi, Honggang;Cooper, Sam F.;Cote, Michel G.. And the article was included in Journal of Chromatography, Biomedical Applications in 1992.Application of 1458-01-1 This article mentions the following:
A simple and sensitive high-performance liquid chromatog. method was developed to screen and determine amiloride (I) in human urine. The detection limit of the method is 0.12 μg/mL and the recovery of amiloride from urine was 80.4-85.5% at different concentration The coefficients of variation were less than 2.8 and 4.4% for intra- and inter-assays, resp. Total urinary excretion of I in 24 h after oral administration of 5 mg or 15 mg of I ranged from 22.0 to 33.3% of the total dose for three different subjects. I could be detected in urine up to at least 44 h after a 5-mg dose and 72 h after a 15-mg dose. A gas chromatog.-mass spectrometric (GC-MS) confirmatory method was established based on the methanolysis of I to Me 3,5-diamino-6-chloropyrazinecarboxylate (II). The di-N-trimethylsilyl derivative of II showed very good GC-MS properties and provided reliable structure information for confirmation anal. of I. This is the first time that a reliable GC-MS method has been reported for the detection of urinary I. In the experiment, the researchers used many compounds, for example, Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate (cas: 1458-01-1Application of 1458-01-1).
Methyl 3,5-diamino-6-chloropyrazine-2-carboxylate (cas: 1458-01-1) belongs to pyrazine derivatives. Pyrazines are part of several biologically active polycyclic compounds; examples are quinoxalines, phenazines; and the bio-luminescent natural products pteridines, flavins, and their derivatives. Pyrazine and a variety of alkylpyrazines are flavor and aroma compounds found in baked and roasted goods. Tetramethylpyrazine (also known as ligustrazine) is reported to scavenge superoxide anion and decrease nitric oxide production in human Granulocytes.Application of 1458-01-1