Month: November 2022

Aulestia, Francisco J.; Webb, Sarah E.; Chan, Ching Man; Tse, Man Kit; Neant, Isabelle; Moreau, Marc; Miller, Andrew L.; Leclerc, Catherine published an article in 2021, the title of the article was A simple bioluminescence-based method for recording Ca2+ signaling in the cytosol or mitochondria of neural progenitor cells from the adult zebrafish brain.Synthetic Route of 55779-48-1 And the article contains the following content:

Neurospheres derived from the adult zebrafish brain are a convenient model for investigating the initial stages of neurogenesis. Neurospheres are composed of stem cells, which have the ability to either self-renew or differentiate into specialized cells (i.e., neurons and glial cells) if cultured in an appropriate medium. It has previously been suggested that Ca2+ signaling in the cytosol and mitochondria might play some role in neurogenesis. Ca2+ signals can be recorded using the non-toxic bioluminescent Ca2+ reporter complex, holoaequorin. We have developed a novel electroporation protocol to load neurospheres with cDNA encoding either mitochondrial-targeted EGFP-apoaequorin (MitGA) or the cytosolic-targeted EGFP-apoaequorin (CytGA). We show that within ∼24 h after electroporation, each of these apoaequorin proteins were expressed in ∼20% of the neurosphere cells. In addition, after reconstitution of holoaequorin by incubation of the cells with its luminophore, coelenterazine, dynamic changes in [Ca2+] in the cytosol and mitochondria were imaged using a custom-built electron multiplying charge coupled device (EMCCD)-based photon imaging microscope (PIM) system or measured using a photomultiplier tube (PMT)-based luminescence detection system, after activation of store-operated Ca2+ entry (SOCE). The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1

The Article related to calcium adult zebrafish brain cytosol mitochondria neural progenitor cell, Placeholder for records without volume info and other aspects.Synthetic Route of 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Mizui, Yuki; Eguchi, Masatoshi; Tanaka, Masanobu; Ikeda, Yuma; Yoshimura, Hideaki; Ozawa, Takeaki; Citterio, Daniel; Hiruta, Yuki published an article in 2021, the title of the article was Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues.Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one And the article contains the following content:

Bioluminescence is a powerful imaging modality for monitoring biol. phenomena both in vitro and in vivo. Bioluminescence imagin (BLI) is becoming a seamless imaging technol. covering the range from cells to organs of small animals. Long-term imaging at the single cell level would lead to a true understanding of the dynamics of life phenomena. This work presents a long-term single cell bioluminescence imaging technol. accomplished with C-3 position protected furimazines (FMZs), a CTZ analogs, which generate intense blue emission when paired with a highly stable engineered luciferase, Nanoluc. Four types of FMZs protected at the C-3 position have been synthesized. The type and steric bulkiness of the protection group strongly contributed to storage stability and the kinetics of the bioluminescence reactions of the analogs in human living cells. In particular, two developed FMZ analogs resulted in significantly longer bioluminescence emission with higher S/N ratio than FMZ at single cell level. Long-term bioluminescence single cell imaging technol. with the developed FMZ analogs will lead to seamless imaging in the range from cells to organs of small animals. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

The Article related to bioluminescence imaging coelenterazine storage stability reaction kinetics, Placeholder for records without volume info and other aspects.Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

O′Sullivan, Justin J.; Medici, Valentina; Heffern, Marie C. published an article in 2022, the title of the article was A caged imidazopyrazinone for selective bioluminescence detection of labile extracellular copper(II).Category: pyrazines And the article contains the following content:

Copper is an essential redox-active metal that plays integral roles in biol. ranging from enzymic catalysis to mitochondrial respiration. However, if not adequately regulated, this redox activity has the potential to cause oxidative stress through the production of reactive oxygen species. Indeed, the dysregulation of copper has been associated with a variety of disease states including diabetes, neurodegenerative disorders, and multiple cancers. While increasing tools are being developed for illuminating labile intracellular copper pools and the trafficking pathways in which they are involved, significantly less attention has been given to the analogus extracellular labile pool. To address this gap, we have developed a bioluminescence-based imaging probe, picolinic ester caged-diphenylterazine (pic-DTZ) for monitoring labile, extracellular copper using a coelenterazine-like imidazopyrazinone and the genetically-engineered, marine-based luciferase, nanoluciferase. Unlike the more commonly-used firefly luciferase, nanoluciferase does not require ATP, allowing its application to the extracellular milieu. pic-DTZ demonstrates high metal and oxidation state selectivity for Cu(II) in aqueous buffer as well as selectivity for labile pools over coordinatively inaccessible protein-bound Cu(II). We demonstrate the potential of pic-DTZ as a diagnostic tool in human serum and plasma for copper-associated diseases. Addnl., we apply pic-DTZ to lend insight into the extracellular copper dynamic in anticancer treatments. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines

The Article related to extracellular copper imidazopyrazinone selective bioluminescence detection, Placeholder for records without volume info and other aspects.Category: pyrazines

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On May 1, 2020, Ikeda, Yuma; Tanaka, Masanobu; Nishihara, Ryo; Hiruta, Yuki; Citterio, Daniel; Suzuki, Koji; Niwa, Kazuki published an article.Synthetic Route of 55779-48-1 The title of the article was Quantitative evaluation of luminescence intensity from enzymatic luminescence reaction of coelenterazine and analogues. And the article contained the following:

Recent advances in bioluminescence technol. rely on the discovery of luciferin-luciferase pairs having excellent luminescence activity. Here, we focus on coelenterazine (CTZ) and its analogs and quant. investigate reaction factors, including bioluminescence reaction quantum yield (φBL) and enzyme reaction kinetics (turnover rate, kcat), determining the luminescence intensity to design brighter reaction systems. Initially, it was confirmed that the CTZ was kept stable during the storage and the φBL value measuring experiment in terms of comparative study using benzyl-protected CTZ. Using a luminometer whose absolute responsivity is calibrated for each luminescence reaction spectrum, we absolutely measured φBL and other reaction factors of CTZ and 3 types of blue-shifted CTZ analogs, CTZ 400a, 6-pi-H-CTZ and 6-pi-Ph-CTZ. The results suggested that there was no significant difference in reaction kinetics, but a clear difference in φBL (up to 22-fold), which was presumably due to an improvement in fluorescence quantum yield (φFL) of the corresponding coelenteramide (CTMD), oxidized form of CTZ. Our evaluation suggests that the design of CTZ analogs considering φFL is important for developing a bright luminescent reaction system. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1

The Article related to tert butoxycarbonyl coelenterazine bioluminescence spectrum charge coupled device, Placeholder for records without volume info and other aspects.Synthetic Route of 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On December 16, 2020, Nishihara, Ryo; Niwa, Kazuki; Tomita, Tatsunosuke; Kurita, Ryoji published an article.Category: pyrazines The title of the article was Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence. And the article contained the following:

A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an ELISA without the need for any sample pretreatments because of the high specificity of HuLumino1. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines

The Article related to coelenterazine analog serum albumin bioluminescence, Biochemical Methods: Spectral and Related Methods and other aspects.Category: pyrazines

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On June 2, 2020, Moutsiopoulou, Angeliki; Broyles, David; Joda, Hamdi; Dikici, Emre; Kaur, Avinash; Kaifer, Angel; Daunert, Sylvia; Deo, Sapna K. published an article.Synthetic Route of 55779-48-1 The title of the article was Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. And the article contained the following:

Although bioluminescent mol. beacons designed around resonance quenchers have shown higher signal-to-noise ratios and increased sensitivity compared with fluorescent beacon systems, bioluminescence quenching is still comparatively inefficient. A more elegant solution to inefficient quenching can be realized by designing a competitive inhibitor that is structurally very similar to the native substrate, resulting in essentially complete substrate exclusion. In this work, the authors designed a conjugated anti-interferon-γ (IFN-γ) mol. aptamer beacon (MAB) attached to a bioluminescent protein, Gaussia luciferase (GLuc), and an inhibitor mol. with a similar structure to the native substrate coelenterazine. To prove that a MAB can be more sensitive and have a better signal-to-noise ratio, a bioluminescence-based assay was developed against IFN-γ and provided an optimized, physiol. relevant detection limit of 1.0 nM. The authors believe that this inhibitor approach may provide a simple alternative strategy to standard resonance quenching in the development of high-performance mol. beacon-based biosensing systems. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1

The Article related to bioluminescence protein inhibitor aptamer beacon biosensor, Biochemical Methods: Spectral and Related Methods and other aspects.Synthetic Route of 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On May 30, 2013, Sawa, Tadaki; Kawabata, Wataru; Asami, Saiko published a patent.HPLC of Formula: 87486-34-8 The title of the patent was Novel fluorescent probe, and kinase inhibitor novel screening method. And the patent contained the following:

A novel benzoxadiazole fluorescent probe shown by the formula (I) is provided, which enables to screen a kinase inhibitor. In (I), Z represents a compound possessing a kinase inhibitory activity; L represents an optionally bound or substituted amino group, a carbonyl group, an optionally substituted hydrocarbon group, an optionally substituted alkylcarbamoyl group, an optionally substituted acylamino group, a sulfide group, or an optionally substituted alkoxy group; X represents an optionally bound or substituted amino group, an optionally substituted hydrazino group, or a thiocarbonyl amino group; and R1 represents a nitro group, or a dimethylsulfamoyl group. The experimental process involved the reaction of 3,5-Dibromo-1-methylpyrazin-2(1H)-one(cas: 87486-34-8).HPLC of Formula: 87486-34-8

The Article related to benzoxadiazole fluorescent probe kinase inhibitor screening, Biochemical Methods: Spectral and Related Methods and other aspects.HPLC of Formula: 87486-34-8

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On December 27, 2019, Klein, William P.; Diaz, Sebastian A.; Chiriboga, Matthew; Walper, Scott A.; Medintz, Igor L. published an article.Recommanded Product: 55779-48-1 The title of the article was Dendrimeric DNA-Based Nanoscaffolded BRET-FRET Optical Encryption Keys. And the article contained the following:

New types of unconventional encryption keys based on phys. unclonable functions are critically needed for information security. Here we prototype such a key based on the (bio)photonic interactions of multiple donor-acceptor dyes and bioluminescent enzymes as assembled on a dendrimeric DNA nanoscaffold. The ability of this optical key to generate ∼1037 unique challenge key combinations utilizing both bioluminescence resonance energy transfer and Förster resonance energy transfer-based interrogation was demonstrated, highlighting how unique codes can be generated and exchanged by different users as shared secret keys. We discuss how alterations in dye number, placement, excitation wavelength, etc. can alter code properties in unpredictable ways. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Recommanded Product: 55779-48-1

The Article related to dendrimeric dna nanoscaffold bret fret optical encryption key, Biochemical Methods: Spectral and Related Methods and other aspects.Recommanded Product: 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

On December 31, 2020, Galaway, Francis; Wright, Gavin J. published an article.Related Products of 55779-48-1 The title of the article was Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase. And the article contained the following:

Extracellular protein interactions mediated by cell surface receptors are essential for intercellular communication in multicellular organisms. Assays to detect extracellular interactions must account for their often weak binding affinities and also the biochem. challenges in solubilizing membrane-embedded receptors in an active form. Methods based on detecting direct binding of soluble recombinant receptor ectodomains have been successful, but genome-scale screening is limited by the usual requirement of producing sufficient amounts of each protein in two different forms, usually a “bait” and “prey”. Here, we show that oligomeric receptor ectodomains coupled to concatenated units of the light-generating Gaussia luciferase enzyme robustly detected low affinity interactions and reduced the amount of protein required by several orders of magnitude compared to other reporter enzymes. Importantly, we discovered that this flash-type luciferase exhibited a reaction-induced inhibition that permitted the use of a single protein preparation as both bait and prey thereby halving the number of expression plasmids and recombinant proteins required for screening. This approach was tested against a benchmarked set of quantified extracellular interactions and shown to detect extremely weak interactions (KDs ≥ μM). This method will facilitate large-scale receptor interaction screening and contribute to the goal of mapping networks of cellular communication. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Related Products of 55779-48-1

The Article related to modified avexis low affinity ectodomain interaction luminescent screening, Biochemical Methods: Spectral and Related Methods and other aspects.Related Products of 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Silva, Jose Pedro; Gonzalez-Berdullas, Patricia; Esteves da Silva, Joaquim C. G.; Pinto da Silva, Luis published an article in 2022, the title of the article was Development of a Coelenterazine Derivative with Enhanced Superoxide Anion-Triggered Chemiluminescence in Aqueous Solution.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one And the article contains the following content:

Superoxide anion is a reactive oxygen species (ROS) of biol. interest. More specifically, it plays a role in intra- and intercellular signaling, besides being associated with conditions such as inflammation and cancer. Given this, efforts have been made by the research community to devise new sensing strategies for this ROS species. Among them, the chemiluminescent reaction of marine Coelenterazine has been employed as a sensitive and dynamic probing approach. Nevertheless, chemiluminescent reactions are typically associated with lower emissions in aqueous solutions Herein, here we report the synthesis of a new Coelenterazine derivative with the potential for superoxide anion sensing. Namely, this novel compound is capable of chemiluminescence in a dose-dependent manner when triggered by this ROS species. More importantly, the light-emission intensities provided by this derivative were relevantly enhanced (intensities 2.13 x 101 to 1.11 x 104 times higher) in aqueous solutions at different pH conditions when compared to native Coelenterazine. The half-life of the chemiluminescent signal is also greatly increased for the derivative Thus, a new chemiluminescence mol. with significant potential for superoxide anion sensing was discovered and reported for the first time. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

The Article related to chemiluminescence coelenterazine imidazopyrazinone derivative ros indicator, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem