Jones, Marieke K. published the artcileDifferential expression of urinary volatile organic compounds by sex, male reproductive status, and pairing status in the maned wolf (Chrysocyon brachyurus), Category: pyrazines, the main research area is Chrysocyon brachyurus urinary volatile organic compound male reproductive system.
The maned wolf (Chrysocyon brachyurus) is an induced ovulator. Though the mechanism of ovulation induction remains unknown, it is suspected to be urinary chem. signals excreted by males. This study assessed volatile organic compounds (VOCs) in weekly urine samples across 5 mo from 13 maned wolves (6 intact males, 1 neutered male, 6 females) with the goal of identifying VOCs that are differentially expressed across sex, reproductive status, and pairing status. Solid-phase microextraction (SPME) and gas chromatog.-mass spectrometry (GC-MS) were used to extract and sep. VOCs that were identified via spectral matching with authentic standards, with spectral libraries, or with new software that further matches mol. fragment structures with mass spectral peaks. Two VOCs were present across all 317 urine samples: 2,5-di-Me pyrazine and 2-methyl-6-(1-propenyl)-pyrazine. Fifteen VOCs differed significantly (Adj). P < 0.001 and |log2 fold change| >2.0 between intact males and females. Using partial least squares-discriminant anal., the compounds with the highest importance to the sex classification were delta-decalactone, delta-dodecalactone, and bis(prenyl) sulfide. Sixty-two VOCs differed between intact males and the neutered male. Important classifier compounds were 3-Et 2,5-di-Me pyrazine, 2-methyl-6-(1-propenyl)-pyrazine, and tetrahydro-2-isopentyl-5-Pr furan. Several VOCs established as important here have been implicated in reproductive communication in other mammals. This study is the most robust examination of differential expression in the maned wolf thus far and provides the most comprehensive anal. of maned wolf urinary VOCs to date, increasing the sample size substantially over previous chem. communication studies in this species. New data anal. software allowed for the identification of compounds in the hormone-producing mevalonate pathway which were previously unreported in maned wolf urine. Several putative semiochems. were identified as good candidates for behavioral bioassays to determine their role in maned wolf reproduction, and specifically in ovulation induction.
PLoS One published new progress about Chrysocyon brachyurus. 14667-55-1 belongs to class pyrazines, name is 2,3,5-Trimethylpyrazine, and the molecular formula is C7H10N2, Category: pyrazines.