Nishihara, Ryo; Abe, Masahiro; Suzuki, Koji; Kim, Sung-Bae published an article in 2021, the title of the article was Luciferase-Specific Coelenterazine Analogues for Optical Cross Talk-Free Bioassays.Category: pyrazines And the article contains the following content:
Spectral overlaps in fluorescence (FL) and bioluminescence (BL) commonly cause optical cross talks. The present protocol introduces five different lineages of coelenterazine (CTZ) analogs, which have selectivity to a specific luciferase, and thus cross talk-free. For example, some CTZ analogs with ethynyl or styryl groups display dramatically biased BL to specific luciferases and pH by modifying the functional groups at the C-2 and C-6 positions of the imidazopyridinne backbone of CTZ. The optical cross talk-free feature is exemplified with the multiplex system, which simultaneously illuminated antiestrogenic and rapamycin activities without optical cross talks. This unique protocol contributes to specific and high-throughput BL imaging of multiple optical readouts in mammalian cells without optical contamination. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines
The Article related to alkynes: chemistry, animals, chlorocebus aethiops, cos cells, estrogen receptor modulators: pharmacology, imidazoles: chemistry, immunosuppressive agents: pharmacology, luciferases: drug effects, luciferases: metabolism, luminescent agents: chemistry, luminescent measurements: methods, optical imaging: methods, pyrazines: chemistry and other aspects.Category: pyrazines