Mizui, Yuki; Eguchi, Masatoshi; Tanaka, Masanobu; Ikeda, Yuma; Yoshimura, Hideaki; Ozawa, Takeaki; Citterio, Daniel; Hiruta, Yuki published an article in 2021, the title of the article was Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues.Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one And the article contains the following content:
Bioluminescence is a powerful imaging modality for monitoring biol. phenomena both in vitro and in vivo. Bioluminescence imagin (BLI) is becoming a seamless imaging technol. covering the range from cells to organs of small animals. Long-term imaging at the single cell level would lead to a true understanding of the dynamics of life phenomena. This work presents a long-term single cell bioluminescence imaging technol. accomplished with C-3 position protected furimazines (FMZs), a CTZ analogs, which generate intense blue emission when paired with a highly stable engineered luciferase, Nanoluc. Four types of FMZs protected at the C-3 position have been synthesized. The type and steric bulkiness of the protection group strongly contributed to storage stability and the kinetics of the bioluminescence reactions of the analogs in human living cells. In particular, two developed FMZ analogs resulted in significantly longer bioluminescence emission with higher S/N ratio than FMZ at single cell level. Long-term bioluminescence single cell imaging technol. with the developed FMZ analogs will lead to seamless imaging in the range from cells to organs of small animals. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one
The Article related to bioluminescence imaging coelenterazine storage stability reaction kinetics, Placeholder for records without volume info and other aspects.Quality Control of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one