On May 1, 2020, Ikeda, Yuma; Tanaka, Masanobu; Nishihara, Ryo; Hiruta, Yuki; Citterio, Daniel; Suzuki, Koji; Niwa, Kazuki published an article.Synthetic Route of 55779-48-1 The title of the article was Quantitative evaluation of luminescence intensity from enzymatic luminescence reaction of coelenterazine and analogues. And the article contained the following:
Recent advances in bioluminescence technol. rely on the discovery of luciferin-luciferase pairs having excellent luminescence activity. Here, we focus on coelenterazine (CTZ) and its analogs and quant. investigate reaction factors, including bioluminescence reaction quantum yield (φBL) and enzyme reaction kinetics (turnover rate, kcat), determining the luminescence intensity to design brighter reaction systems. Initially, it was confirmed that the CTZ was kept stable during the storage and the φBL value measuring experiment in terms of comparative study using benzyl-protected CTZ. Using a luminometer whose absolute responsivity is calibrated for each luminescence reaction spectrum, we absolutely measured φBL and other reaction factors of CTZ and 3 types of blue-shifted CTZ analogs, CTZ 400a, 6-pi-H-CTZ and 6-pi-Ph-CTZ. The results suggested that there was no significant difference in reaction kinetics, but a clear difference in φBL (up to 22-fold), which was presumably due to an improvement in fluorescence quantum yield (φFL) of the corresponding coelenteramide (CTMD), oxidized form of CTZ. Our evaluation suggests that the design of CTZ analogs considering φFL is important for developing a bright luminescent reaction system. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1
The Article related to tert butoxycarbonyl coelenterazine bioluminescence spectrum charge coupled device, Placeholder for records without volume info and other aspects.Synthetic Route of 55779-48-1