On April 30, 2021, Giuliani, Germano; Merolla, Assunta; Paolino, Marco; Reale, Annalisa; Saletti, Mario; Blancafort, Lluis; Cappelli, Andrea; Benfenati, Fabio; Cesca, Fabrizia published an article.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one The title of the article was Stability Studies of New Caged bis-deoxy-coelenterazine Derivatives and Their Potential Use as Cellular pH Probes. And the article contained the following:
The synthesis of new bis-deoxy-coelenterazine (1) derivatives bearing ester protective groups (acetate, propionate and butyrate esters) was accomplished. Moreover, their hydrolytic stability at room temperature was evaluated in dimethylsulfoxide (DMSO) as solvent, using the NMR (NMR) spectra of the key products at different time intervals. The results showed an increasing hydrolysis rate according to longest aliphatic chain, with a half-life of 24 days of the more stable acetate derivative (4a). Furthermore, the anal. of the exptl. data revealed the greater stability of the enol tautomer in this aprotic polar solvent. This result was confirmed by theor. calculations using the d. functional theory (DFT) approach, which gave us the opportunity to propose a detailed decomposition mechanism. Addnl., the derivatives obtained were tested by bioluminescence luciferase assays to evaluate their potential use as extracellular pH-sensitive reporter substrates of luciferase. The biol. data support the idea that further structural modifications of these mols. may open promising perspectives in this field of research. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one
The Article related to bis deoxy coelenterazine derivative ph probe stability, Placeholder for records without volume info and other aspects.Reference of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one