On August 16, 2022, Cumberbatch, Derrick; Mori, Tetsuya; Yang, Jie; Mi, Dehui; Vinson, Paige; Weaver, C. David; Johnson, Carl Hirschie published an article.Computed Properties of 55779-48-1 The title of the article was A BRET Ca2+ sensor enables high-throughput screening in the presence of background fluorescence. And the article contained the following:
The intrinsic fluorescence of samples confounds the use of fluorescence-based sensors. This is of particular concern in high-throughput screening (HTS) applications using large chem. libraries containing intrinsically fluorescent compounds To overcome this problem, we developed a bioluminescence resonance energy transfer (BRET) Ca2+ sensor, CalfluxCTN. We demonstrated that it reliably reported changes in intracellular Ca2+ concentrations evoked by an agonist and an antagonist of the human muscarinic acetylcholine receptor M1 (hM1R) even in the presence of the fluorescent compound fluorescein, which interfered with a standard fluorescent HTS sensor (Fluo-8). In an HTS using a chem. library containing fluorescent compounds, CalfluxCTN accurately identified agonists and antagonists that were missed or miscategorized using Fluo-8. Moreover, we showed that a luciferase substrate that becomes activated only when inside cells generated long-lasting BRET signals in HTS, enabling results to be reliably compared among replicate samples for hours. Thus, the use of a self-luminescent sensor instead of a fluorescent sensor could facilitate the complete screening of chem. libraries in a high-throughput context and enable anal. of autofluorescent samples in many different applications. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Computed Properties of 55779-48-1
The Article related to bioluminescence resonance energy transfer sensor fluorescence screening, Placeholder for records without volume info and other aspects.Computed Properties of 55779-48-1