On June 2, 2020, Moutsiopoulou, Angeliki; Broyles, David; Joda, Hamdi; Dikici, Emre; Kaur, Avinash; Kaifer, Angel; Daunert, Sylvia; Deo, Sapna K. published an article.Synthetic Route of 55779-48-1 The title of the article was Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. And the article contained the following:
Although bioluminescent mol. beacons designed around resonance quenchers have shown higher signal-to-noise ratios and increased sensitivity compared with fluorescent beacon systems, bioluminescence quenching is still comparatively inefficient. A more elegant solution to inefficient quenching can be realized by designing a competitive inhibitor that is structurally very similar to the native substrate, resulting in essentially complete substrate exclusion. In this work, the authors designed a conjugated anti-interferon-γ (IFN-γ) mol. aptamer beacon (MAB) attached to a bioluminescent protein, Gaussia luciferase (GLuc), and an inhibitor mol. with a similar structure to the native substrate coelenterazine. To prove that a MAB can be more sensitive and have a better signal-to-noise ratio, a bioluminescence-based assay was developed against IFN-γ and provided an optimized, physiol. relevant detection limit of 1.0 nM. The authors believe that this inhibitor approach may provide a simple alternative strategy to standard resonance quenching in the development of high-performance mol. beacon-based biosensing systems. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Synthetic Route of 55779-48-1
The Article related to bioluminescence protein inhibitor aptamer beacon biosensor, Biochemical Methods: Spectral and Related Methods and other aspects.Synthetic Route of 55779-48-1