October 2022 - Page 8 of 14

Hasgur, Suheyla et al. published their research in Cytotherapy in 2021 |CAS: 55779-48-1

The Article related to mesenchymal stromal cell splenic macrophage phagocytosis tumor localization, cancer cell therapy, lentiviral transduction, mesenchymal stromal cells (mscs), phagocytosis, splenic macrophage, stem cell transplantation, tumor homing and other aspects.SDS of cas: 55779-48-1

On May 31, 2021, Hasgur, Suheyla; Desbourdes, Laura; Relation, Theresa; Overholt, Kathleen M.; Stanek, Joseph R.; Guess, Adam J.; Yu, Minjun; Patel, Pratik; Roback, Linda; Dominici, Massimo; Otsuru, Satoru; Horwitz, Edwin M. published an article.SDS of cas: 55779-48-1 The title of the article was Splenic macrophage phagocytosis of intravenously infused mesenchymal stromal cells attenuates tumor localization. And the article contained the following:

Mesenchymal stromal cells (MSCs) possess remarkable tumor tropism, making them ideal vehicles to deliver tumor-targeted therapeutic agents; however, their value in clin. medicine has yet to be realized. A barrier to clin. utilization is that only a small fraction of infused MSCs ultimately localize to the tumor. In an effort to overcome this obstacle, we sought to enhance MSC trafficking by focusing on the factors that govern MSC arrival within the tumor microenvironment. Our findings show that MSC chemoattraction is only present in select tumors, including osteosarcoma, and that the chemotactic potency among similar tumors varies substantially. Using an osteosarcoma xenograft model, we show that human MSCs traffic to the tumor within several hours of infusion. After arrival, MSCs are observed to localize in clusters near blood vessels and MSC-associated bioluminescence signal intensity is increased, suggesting that the seeded cells expand after engraftment. However, our studies reveal that a significant portion of MSCs are eliminated en route by splenic macrophage phagocytosis, effectively limiting the number of cells available for tumor engraftment. To increase MSC survival, we transiently depleted macrophages with liposomal clodronate, which resulted in increased tumor localization without substantial reduction in tumor-associated macrophages. Our data suggest that transient macrophage depletion will significantly increase the number of MSCs in the spleen and thus improve MSC localization within a tumor, theor. increasing the ED of an anti-cancer agent. This strategy may subsequently improve the clin. efficacy of MSCs as vehicles for the tumor-directed delivery of therapeutic agents. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).SDS of cas: 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Kim, Hyemin et al. published their research in Advanced Science (Weinheim, Germany) in 2022 |CAS: 55779-48-1

The Article related to luciferase encapsulated polymersome artificial organelle optogenetic modulation cardiomyocyte, artificial organelles, bioluminescences, cardiomyocytes, nanoreactors, optogenetics, polymerization-induced self-assembly, polymersomes and other aspects.HPLC of Formula: 55779-48-1

On September 23, 2022, Kim, Hyemin; Yeow, Jonathan; Najer, Adrian; Kit-Anan, Worrapong; Wang, Richard; Rifaie-Graham, Omar; Thanapongpibul, Chalaisorn; Stevens, Molly M. published an article.HPLC of Formula: 55779-48-1 The title of the article was Microliter Scale Synthesis of Luciferase-Encapsulated Polymersomes as Artificial Organelles for Optogenetic Modulation of Cardiomyocyte Beating. And the article contained the following:

Constructing artificial systems that effectively replace or supplement natural biol. machinery within cells is one of the fundamental challenges underpinning bioengineering. At the sub-cellular scale, artificial organelles (AOs) have significant potential as long-acting biomedical implants, mimicking native organelles by conducting intracellularly compartmentalized enzymic actions. The potency of these AOs can be heightened when judiciously combined with genetic engineering, producing highly tailorable biohybrid cellular systems. Here, the authors present a cost-effective, microliter scale (10 μL) polymersome (PSome) synthesis based on polymerization-induced self-assembly for the in situ encapsulation of Gaussia luciferase (GLuc), as a model luminescent enzyme. These GLuc-loaded PSomes present ideal features of AOs including enhanced enzymic resistance to thermal, proteolytic, and intracellular stresses. To demonstrate their biomodulation potential, the intracellular luminescence of GLuc-loaded PSomes is coupled to optogenetically engineered cardiomyocytes, allowing modulation of cardiac beating frequency through treatment with coelenterazine (CTZ) as the substrate for GLuc. The long-term intracellular stability of the luminescent AOs allows this cardiostimulatory phenomenon to be reinitiated with fresh CTZ even after 7 days in culture. This synergistic combination of organelle-mimicking synthetic materials with genetic engineering is therefore envisioned as a highly universal strategy for the generation of new biohybrid cellular systems displaying unique triggerable properties. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).HPLC of Formula: 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Al-Ani, Ali W. et al. published their research in Nanomedicine (New York, NY, United States) in 2019 |CAS: 55779-48-1

The Article related to luciferase zinc protoporphyrin bioluminescence photodynamic therapy breast cancer, bioluminescence resonance energy transfer, gaussia princeps luciferase, listeria innocua dna binding protein, photodynamic therapy, zinc (ii) protoporphyrin ix and other aspects.SDS of cas: 55779-48-1

On August 31, 2019, Al-Ani, Ali W.; Zhang, Lei; Ferreira, Lenny; Turyanska, Lyudmila; Bradshaw, Tracey D.; Thomas, Neil R. published an article.SDS of cas: 55779-48-1 The title of the article was Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX. And the article contained the following:

Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chem. conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).SDS of cas: 55779-48-1

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Vicente, Manuel et al. published their research in International Journal of Molecular Sciences in 2019 |CAS: 55779-48-1

The Article related to danio calcium mitochondria skeletal muscle bioluminescent indicator gfp aequorin, gfp-aequorin, twitch-4, aequorin, bioluminescence, calcium, genetically encoded calcium indicator (geci), microscopy, mitochondria, skeletal muscle, zebrafish embryo and other aspects.Application In Synthesis of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

Vicente, Manuel; Salgado-Almario, Jussep; Soriano, Joaquim; Burgos, Miguel; Domingo, Beatriz; Llopis, Juan published an article in 2019, the title of the article was Visualization of mitochondrial Ca2+ signals in skeletal muscle of zebrafish embryos with bioluminescent indicators.Application In Synthesis of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one And the article contains the following content:

Mitochondria are believed to play an important role in shaping the intracellular Ca2+ transients during skeletal muscle contraction. There is discussion about whether mitochondrial matrix Ca2+ dynamics always mirror the cytoplasmic changes and whether this happens in vivo in whole organisms. In this study, we characterized cytosolic and mitochondrial Ca2+ signals during spontaneous skeletal muscle contractions in zebrafish embryos expressing bioluminescent GFP-aequorin (GA, cytoplasm) and mitoGFP-aequorin (mitoGA, trapped in the mitochondrial matrix). The Ca2+ transients measured with GA and mitoGA reflected contractions of the trunk observed by transmitted light. The mitochondrial uncoupler FCCP and the inhibitor of the mitochondrial calcium uniporter (MCU), DS16570511, abolished mitochondrial Ca2+ transients whereas they increased the frequency of cytosolic Ca2+ transients and muscle contractions, confirming the subcellular localization of mitoGA. Mitochondrial Ca2+ dynamics were also determined with mitoGA and were found to follow closely cytoplasmic changes, with a slower decay. Cytoplasmic Ca2+ kinetics and propagation along the trunk and tail were characterized with GA and with the genetically encoded fluorescent Ca2+ indicator, Twitch-4. Although fluorescence provided a better spatio-temporal resolution, GA was able to resolve the same kinetic parameters while allowing continuous measurements for hours. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Application In Synthesis of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Azad, Taha et al. published their research in Molecular Therapy in 2021 |CAS: 55779-48-1

The Article related to ace2 spike glycoprotein interaction glycosylation sars cov2 covid19 human, sarscov2 s protein viral entry nanoluciferase bioreporter n glycosylation, 2019-ncov, covid-19, sars-cov-2, bioluminescence, bioreporter, coronavirus, high-throughput screening, viral entry and other aspects.Formula: C26H21N3O3

On June 2, 2021, Azad, Taha; Singaravelu, Ragunath; Taha, Zaid; Jamieson, Taylor R.; Boulton, Stephen; Crupi, Mathieu J. F.; Martin, Nikolas T.; Brown, Emily E. F.; Poutou, Joanna; Ghahremani, Mina; Pelin, Adrian; Nouri, Kazem; Rezaei, Reza; Marshall, Christopher Boyd; Enomoto, Masahiro; Arulanandam, Rozanne; Alluqmani, Nouf; Samson, Reuben; Gingras, Anne-Claude; Cameron, D. William; Greer, Peter A.; Ilkow, Carolina S.; Diallo, Jean-Simon; Bell, John C. published an article.Formula: C26H21N3O3 The title of the article was Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry. And the article contained the following:

The ongoing COVID-19 pandemic has highlighted the immediate need for the development of antiviral therapeutics targeting different stages of the SARS-CoV-2 life cycle. We developed a bioluminescence-based bioreporter to interrogate the interaction between the SARS-CoV-2 viral spike (S) protein and its host entry receptor, angiotensin-converting enzyme 2 (ACE2). The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, resp. Using this bioreporter, we uncovered critical host and viral determinants of the interaction, including a role for glycosylation of asparagine residues within the RBD in mediating successful viral entry. We also demonstrate the importance of N-linked glycosylation to the RBD’s antigenicity and immunogenicity. Our study demonstrates the versatility of our bioreporter in mapping key residues mediating viral entry as well as screening inhibitors of the ACE2-RBD interaction. Our findings point toward targeting RBD glycosylation for therapeutic and vaccine strategies against SARS-CoV-2. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Formula: C26H21N3O3

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Tunes, Luiza Guimaraes’s team published research in African Journal of Microbiology Research in 2019 | 2873-36-1

African Journal of Microbiology Research published new progress about Alkaloids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Product Details of C11H18N2O2.

Tunes, Luiza Guimaraes; Goncalves, Vivian Nicolau; Bueno, Daniela Nabak; Zani, Carlos Leomar; Rosa, Luiz Henrique; Cota, Betania Barros published the artcile< Diketopiperazine alkaloids produced by the endophytic fungus Penicillium citrinum and evaluation of their antileishmanial activity>, Product Details of C11H18N2O2, the main research area is Penicillium citrinum antileishmanial endophytic fungus diketopiperazine alkaloid.

Chromatog. fractionation of the antileishmanial extract obtained from fermentation of the endophytic fungus Penicillium citrinum, isolated from leaves of Ageratum myriadenia, yielded three diketopiperazine alkaloids; cyclo(L-Pro-L-Leu) (1), cyclo-(L-Pro-L-Phe) (2) and tryprostatin B (3). The structures of these compounds were established on the basis of spectroscopic methods and comparison with the literature. Compounds 1 and 2 were active against both amastigote-like forms of Leishmania (Leishmania) amazonensis and intracelular amastigotes of L. (Leishmania) infantum with approx. 50% of parasite growth inhibition at 100μM. None of the compounds were considered toxic against human leukemia monocyte cell line (THP-1) at 100μM. It is the first report about isolation of these diketopiperazines from P. citrinum and their antileishmanial potential against L. (L.) infantum.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Yang, Bo’s team published research in Chemistry of Natural Compounds in 2020-11-30 | 2873-36-1

Chemistry of Natural Compounds published new progress about Bioactive amplification with probing assay. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.

Yang, Bo; Yang, Zhong-Duo; Li, Xiao-Fei; Shu, Zong-Mei published the artcile< Secondary Metabolites of the Endophytic Fungi Talaromyces wortmannii Cultivated in Maize Medium and their Bioactivity>, Synthetic Route of 2873-36-1, the main research area is Talaromyces sitosterol deacetylisowortmin aspergillumarin.

In this paper, we described the isolation and identification of eight compounds (1-8) from an endophytic fungus LGT-4 (identified as Talaromyces wortmannii) of Tripterygium wilfordii. The fungal strain (LGT-4) was isolated from the roots of Tripterygium wilfordii and identified as Talaromyces wortmannii based on both morphol. on PDA and anal. of the DNA sequences of the ITS1-5.8S-ITS2 rRNA gene region. A GenBank search for DNA sequence (GenBank Accession Number KF850714) similarity revealed that ITS1-5.8S-ITS2 of LGT-4 was 99% homologous to that of Talaromyces wortmannii reference strains (GenBank Accession Numbers FR667650.1 and JN807336.1). The strain Lgt-4 was cultivated in maize medium (100 mL × 80 Erlenmeyer flask) for 40 days at 28°C in a constant temperature oscillation incubator. The culture was extracted with EtOAc (8 L) to afford the crude extract (26 g), which was subjected to column chromatog. using silica gel, Sephadex LH-20, MCI-CHP20P gel, and HPLC, etc. A total of eight compounds was isolated and identified based on NMR spectra. To the best of our knowledge, this is the first report on the isolation of the 4, 5, and 7 from Talaromyces wortmannii

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Arndt, Daniel’s team published research in Rapid Communications in Mass Spectrometry in 2020 | 2873-36-1

Rapid Communications in Mass Spectrometry published new progress about Composition. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Quality Control of 2873-36-1.

Arndt, Daniel; Wachsmuth, Christian; Buchholz, Christoph; Bentley, Mark published the artcile< A complex matrix characterization approach, applied to cigarette smoke, that integrates multiple analytical methods and compound identification strategies for non-targeted liquid chromatography with high-resolution mass spectrometry>, Quality Control of 2873-36-1, the main research area is cigarette smoke composition high resolution accurate mass spectrometry.

Rationale : For the characterization of the chem. composition of complex matrixes such as tobacco smoke, containing more than 6000 constituents, several anal. approaches have to be combined to increase compound coverage across the chem. space. Furthermore, the identification of unknown mols. requiring the implementation of addnl. confirmatory tools in the absence of reference standards, such as tandem mass spectrometry spectra comparisons and in silico prediction of mass spectra, is a major bottleneck. Methods : We applied a combination of four chromatog./ionization techniques (reversed-phase (RP) – heated electrospray ionization (HESI) in both pos. (+) and neg. (-) modes, RP – atm. pressure chem. ionization (APCI) in pos. mode, and hydrophilic interaction liquid chromatog. (HILIC) – HESI pos.) using a Thermo Q Exactive® liquid chromatog./high-resolution accurate mass spectrometry (LC/HRAM-MS) platform for the anal. of 3R4F-derived smoke. Compound identification was performed by using mass spectral libraries and in silico predicted fragments from multiple integrated databases. Results : A total of 331 compounds with semi-quant. estimates ≥100 ng per cigarette were identified, which were distributed within the known chem. space of tobacco smoke. The integration of multiple LC/HRAM-MS-based chromatog./ionization approaches combined with complementary compound identification strategies was key for maximizing the number of amenable compounds and for strengthening the level of identification confidence. A total of 50 novel compounds were identified as being present in tobacco smoke. In the absence of reference MS2 spectra, in silico MS2 spectra prediction gave a good indication for compound class and was used as an addnl. confirmatory tool for our integrated non-targeted screening (NTS) approach. Conclusions : This study presents a powerful chem. characterization approach that has been successfully applied for the identification of novel compounds in cigarette smoke. We believe that this innovative approach has general applicability and a huge potential benefit for the anal. of any complex matrixes.

Rapid Communications in Mass Spectrometry published new progress about Composition. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Quality Control of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Wang, Fengwu’s team published research in Natural Product Research in 2021 | 2873-36-1

Natural Product Research published new progress about Alzheimer disease. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Formula: C11H18N2O2.

Wang, Fengwu; Wang, Feng; Chen, Tiejun published the artcile< Secondary metabolites of Galactomyces geotrichum from Laminaria japonica ameliorate cognitive deficits and brain oxidative stress in D-galactose induced Alzheimer′s disease mouse model>, Formula: C11H18N2O2, the main research area is Alzheimer’s disease cognitive deficits brain oxidative stress Galactomyces Laminaria; Alzheimer’s disease; Galactomyces geotrichum; cognitive deficits; oxidative damages; secondary metabolites; toxicological evaluation.

Accumulating evidences have shown the beneficial effects of natural products for Alzheimer′s disease (AD) treatment. The present study was designed to investigate the neuroprotective effects of secondary metabolites of Galactomyces geotrichum (SMGG) on D-galactose induced AD mice. SMGG was extracted and its toxicol. evaluation was conducted. To explore the neuroprotective mechanism responsible for anti-AD activity of SMGG, spatial learning and memory behavioral, oxidative stress levels, acetylcholinesterase and choline acetyltransferase activity assays were employed. The AD mice received SMGG treatment exhibited significant improvement in cognitive performance, enhanced antioxidant capacity, decreased acetylcholinesterase activity and increased choline acetyltransferase activity. Meanwhile, SMGG had no toxicity and seven compounds were separated from it: 7,8-dimethyl-iso-alloxazine, 1-methyl-3-benzyl-6-(4-hydroxybenzyl)-2,5-piperzainedione, cyclo-(Phe-Pro), cyclo-(Leu-Pro), cyclo-(Pro-Gly), cyclo-(Gly-Leu) and uracil, resp. Overall, these data suggested that SMGG protects the brain against D-galactose induced cognitive impairment, oxidative damages and acetylcholine content decrease in AD mice.

Natural Product Research published new progress about Alzheimer disease. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Formula: C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Li, Yuan’s team published research in Journal of Functional Foods in 2020-09-30 | 2873-36-1

Journal of Functional Foods published new progress about Alcohols Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, HPLC of Formula: 2873-36-1.

Li, Yuan; Yuan, Siqi; Yong, Xihao; zhao, Ting; Liu, Jun published the artcile< Research progress on small peptides in Chinese Baijiu>, HPLC of Formula: 2873-36-1, the main research area is review peptide research progress Baijiu.

A review. Chinese Baijiu is a distilled spirit with >100 health promoting compounds produced by diverse microorganisms through the fermentation process. Compared with other distilled spirits in the world, various small peptide compounds in Chinese Baijiu endow it with unparalleled health benefits. In this review, we summarize the small peptides that have been found in Chinese Baijiu, and we provide the unique physiol. and pharmacol. activity of these small peptides by reviewing recent research papers. Existing problems in current research on small peptides in Chinese Baijiu, and the direction and significance of future research are also discussed.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem