Month: October 2022

Parasuraman, Paramanantham; Devadatha, B.; Sarma, V. Venkateswara; Ranganathan, Sampathkumar; Ampasala, Dinakara Rao; Reddy, Dhanasekhar; Kumavath, Ranjith; Kim, In-Won; Patel, Sanjay K. S.; Kalia, Vipin Chandra; Lee, Jung-Kul; Siddhardha, Busi published the artcile< Inhibition of microbial quorum sensing mediated virulence factors by Pestalotiopsis sydowiana>, Formula: C11H18N2O2, the main research area is sequence Pestalotiopsis extract virulence factor quorum sensing inhibitor antipathogenic; Pestalotiopsis sydowiana; Pseudomonas aeruginosa; anti-biofilm; anti-quorum sensing; gene expression; in silico.

Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal (Pestalotiopsis sydowiana PPR) extract was found to possess effective antipathogenic characteristics. The min. inhibitory concentration (MIC) of the fungal extract against test pathogen Pseudomonas aeruginosa PAO1 was 1,000 μg/mL. Sub-MIC concentrations (250 and 500 μg/mL) of fungal extract reduced QSregulated virulence phenotypes such as the production of pyocyanin, chitinase, protease, elastase, and staphylolytic activity in P. aeruginosa PAO1 by 84.15%, 73.15%, 67.37%, 62.37%, and 33.65%, resp. Moreover, it also reduced the production of exopolysaccharides (74.99%), rhamnolipids (68.01%), and alginate (54.98%), and inhibited the biofilm formation of the bacteria by 90.54%. In silico anal. revealed that the metabolite of P. sydowiana PPR binds to the bacterial QS receptor proteins (LasR and RhlR) similar to their resp. natural signaling mols. Cyclo(- Leu-Pro) (CLP) and 4-Hydroxyphenylacetamide (4-HPA) were identified as potent bioactive compounds among the metabolites of P. sydowiana PPR using in silico approaches. The MIC values of CLP and 4-HPA against P. aeruginosa PAO1 were determined as 250 and 125 μg/mL, resp. All the antivirulence assays were conducted at sub-MIC concentrations of CLP (125 μg/mL) and 4-HPA (62.5 μg/mL), which resulted in marked reduction in all the investigated virulence factors. This was further supported by gene expression studies. The findings suggest that the metabolites of P. sydowiana PPR can be employed as promising QS inhibitors that target pathogenic bacteria.

Journal of Microbiology and Biotechnology published new progress about Antibacterial agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Formula: C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Begum Ahil, Sajeli; Hira, Kirti; Shaik, Ameer Basha; Pal, Pragya Paramita; Kulkarni, Onkar Prakash; Araya, Hiroshi; Fujimoto, Yoshinori published the artcile< L-Proline-based-cyclic dipeptides from Pseudomonas sp. (ABS-36) inhibit pro-inflammatory cytokines and alleviate crystal-induced renal injury in mice>, SDS of cas: 2873-36-1, the main research area is sequence Pseudomonas proline cyclic dipeptide renal injury proinflammatory cytokine; Cyclic dipeptides; Cyclo(Val-Pro); IL-1β; IL-6; Oxalate-induced nephropathy; Pseudomonas; Renal inflammation; TNF-α.

Study on the constituents of bioactive culture broth extract (CBE) of Pseudomonas sp. (ABS-36) explored the secretion of an array of cyclic dipeptides (CDPs) and twenty of them had been isolated and reported in the present paper. Six major CDPs [cyclo(Leu-Pro) (1), cyclo(Val-Pro) (2), cyclo(Leu-hydroxy-Pro) (9), cyclo(Pro-Tyr) (10), cyclo(Pro-Ala) (11) and cyclo(Gly-Pro) (12)] exhibited pan cytokine inhibition effect by inhibiting key pro-inflammatory cytokines IL-1β, TNF-α and IL-6 tested under various cell based assays. With this background, the effect of these six CDPs in treating renal inflammation was screened using crystal-induced nephropathy model in mice at 50 mg/kg body weight through oral administration. cis-Cyclo(Val-Pro) (2) exhibited 57% inhibition of plasma IL-1β protein expression and 35.2% inhibition of elevated blood urea nitrogen. Further, cis-cyclo(Val-Pro) (2) attenuated renal injury as demonstrated by significant reduction of mRNA expressions of IL-1β (P < 0.01) and kidney injury marker-1 (P < 0.001). Furthermore, evaluation of tubular-necrosis, -dilation and -cast in the histol. sections exhibited moderate protection of renal tissues by cis-cyclo(Val-Pro) (2). All the tested CDPs reduced the nitrite production and were interestingly non-cytotoxic. The isolated fluorescing bacterial strain was identified as Pseudomonas species by comparison of 16S rDNA sequence and registered as ABS-36 strain GenBank Accession Number KT625586. International Immunopharmacology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Lv, Zhen-Cheng; Zhou, Xiang-Lu; Yan, Jia-Hui; Peng, Yong-Hong; Xu, Liang-Xiong published the artcile< Secondary Metabolites and Antifungal Activity of the Endophytic Fungus Streptomyces humidus SCB0232 from Water Chestnut>, Synthetic Route of 2873-36-1, the main research area is Streptomyces extract antifungal agent Fusarium.

A new macrolide, named concanamycin H (1), together with 12 known compounds (2-13), was isolated from the endophytic Streptomyces humidus SCB0232 for the first time. Compounds 1-13 were identified by anal. of spectroscopic data. Compounds trienomycin A (2) and trienomycin E (3) exhibited moderate inhibitory activities against Fusarium verticillioides and Phytophthora infestans with IC50 values ranging from 32.64 to 72.35 μg·mL-1.

Chemistry of Natural Compounds published new progress about Fungicides. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem