Month: October 2022

Cao, Duc Danh; Trinh, Thi Thanh Van; Mai, Huong Doan Thi; Vu, Van Nam; Le, Hong Minh; Thi, Quyen Vu; Nguyen, Mai Anh; Duong, Thu Trang; Tran, Dang Thach; Chau, Van Minh; Ma, Rui; Shetye, Gauri; Cho, Sanghyun; Murphy, Brian T.; Pham, Van Cuong published the artcile< Antimicrobial lavandulylated flavonoids from a sponge-derived Streptomyces sp. G248 in East Vietnam Sea>, Quality Control of 2873-36-1, the main research area is Streptomyces antimicrobial lavandulylate flavonoid; Streptomyces; actinomycete; anti-microbial; anti-tuberculosis; cytotoxicity; flavonoid.

Three new lavandulylated flavonoids, (2S,2S)-6-lavandulyl-7,4-dimethoxy-5,2-dihydroxylflavanone (1), (2S,2-S)-6-lavandulyl-5,7,2,4-tetrahydroxylflavanone (2), and (2S)-5-lavandulyl-2-methoxy-2,4,4,6-tetrahydroxylchalcone (3), along with seven known compounds 4-10 were isolated from culture broth of Streptomyces sp. G248. Their structures were established by spectroscopic data anal., including 1D and 2D NMR (NMR), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). The absolute configurations of 1-3 were resolved by comparison of their exptl. and calculated electronic CD spectra. Compounds 1-3 exhibited remarkable antimicrobial activity. Whereas, two known compounds 4 and 5 exhibited inhibitory activity against Mycobacterium tuberculosis H37Rv with min. inhibitory concentration (MIC) values of 6.0 μg/mL and 11.1 μg/mL, resp.

Marine Drugs published new progress about Antimicrobial agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Quality Control of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Shen, Shuo; Li, Wei published the artcile< The inhibitory effects of metabolites from Bacillus pumilus on potato virus Y and the induction of early response genes in Nicotiana tabacum>, SDS of cas: 2873-36-1, the main research area is Bacillus Nicotiana metabolites potato virus Y; Active compounds; Early response genes; Halophilic bacterium Bacillus pumilus; Inhibitory activity; Potato Virus Y genes encoding viral proteins.

To develop a new antiviral preparation from a microbial source, the halophilic bacterium Bacillus pumilus E303035 was isolated from a soil sample collected at Qarhan Salt Lake in Qinghai, China. The inhibitory activity of an Et acetate extract of its fermentation broth was higher than that of an n-butanol extract After isolation and purification, 9 compounds were obtained: cyclo(L-Leu-L-Pro) (1), cyclo(L-Pro-L-Tyr) (2), Brevianamide F (3), 2-(3-Indolyl) ethanol (4), N-[2-(1H-indol-3-yl) ethyl] acetamide (5), 3, 3-di(1H-indol-3-yl)propane-1,2-diol (6), Lincomycin B (7), dibutylphthalate (8), and p-hydroxyphenethyl alc. (9). Compounds 1, 5, and 9 showed inhibitory activities against potato virus Y (PVY). Compounds 1, 4, and 9 had significant inhibitory activity against genes HC-pro, P3, and Nib, compound 5 against gene P3, and compounds 1 and 4 against NIa. Compounds 1, 4, 5, and 9 had significant inhibitory activity against genes VPg and 6K1. Active compounds 1, 5, and 9 had various effects on the expression of viral genes related to pathogenesis. Expression of genes cullin and XTH was up-regulated and CP was down-regulated, compared to the pos. control.

AMB Express published new progress about Cullins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Salman, Mahwish; Tariq, Anam; Mustafa, Ghulam; Javed, Muhammad Rizwan; Naheed, Shazia; Qamar, Sarmad Ahmad published the artcile< Cyclo(L-Leucyl-L-Prolyl) from Lactobacillus coryniformis BCH-4 inhibits the proliferation of Aspergillus flavus: an in vitro to in silico approach>, Reference of 2873-36-1, the main research area is Lactobacillus Aspergillus proliferation Cyclo L Leucyl Prolyl antifungal bioprotectant; Antifungal potential; Aspergillus flavus; Bioprotectant; Cyclo(L-Leu-L-Pro); Lactobacillus coryniformis; Molecular docking.

Fungal spoilage led to a considerable economic loss of foodstuff which ultimately affects public health due to mycotoxins production Moreover, the consumption of com. antifungal drugs creates side effects and develops antifungal resistance. To overcome these challenges, the current work was aimed to investigate novel antifungal cyclic dipeptide (CDP) from Lactobacillus coryniformis (Loigolactobacillus coryniformis) BCH-4. CDPs have flexible, cyclic, and stable conformation. The proline-based CDPs provide addnl. structural compatibility and bio-functional values. Keeping in view, high-performance liquid chromatog. (HPLC) was performed to explore cyclo(L-Leu-L-Pro) from L. coryniformis BCH-4. The HPLC detected concentration (135 ± 7.07 mg/mL) exhibited in vitro antifungal activity of 5.66 ± 0.57 mm (inhibitory zone) against Aspergillus flavus. Based on these results, cyclo(L-Leu-L-Pro) was used as a bioprotectant for selected food samples (grapes, lemon, cashew nuts, and almonds). A significant impact of cyclo(L-Leu-L-Pro) was observed in contrast with MRS broth (control) and cell-free supernatant. In silico mol. docking anal. of this CDP was carried out against FAD glucose dehydrogenase, dihydrofolate reductase, and urate oxidase of A. flavus as target proteins. Among these proteins, FAD glucose dehydrogenase exerted strong interactions with cyclo(L-Leu-L-Pro) having S-score of – 8.21. The results evaluated that the detected CDP has strong interactions with selected proteins, causing excellent growth inhibition of A. flavus. Therefore, cyclo(L-Leu-L-Pro) could be used as a potent bioprotectant against food-borne pathogenic fungi.

Archives of Microbiology published new progress about Almond. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Reference of 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Zhu, Junli; Zhang, Yuwei; Deng, Jingmin; Jiang, Hanyun; Zhuang, Liumin; Ye, Wei; Ma, Jiayu; Jiang, Jingyang; Feng, Lifang published the artcile< Diketopiperazines Synthesis Gene in Shewanella baltica and Roles of Diketopiperazines and Resveratrol in Quorum Sensing>, HPLC of Formula: 2873-36-1, the main research area is diketopiperazine resveratrol quorum sensing Shewanella; Shewanella baltica; cyclodipeptide synthase (CDPS); diketopiperazines; quorum sensing; resveratrol.

The synthesis pathways of quorum sensing (QS) signal mols. and the mechanism of action of quorum sensing inhibitors (QSIs) have gained considerable attention as research topics in the field of food preservation. Here, Shewanella baltica was detected as the specific spoilage organism in large yellow croaker during 4° storage, and it produced the QS signal mols. autoinducer-2 (AI-2) and diketopiperazines (DKPs). Then, a cyclodipeptide synthase (CDPS) homologous gene, sb1370, was screened, and knockout and rescue results revealed that this gene was involved in DKP synthesis but not in AI-2 synthesis, and it also played an important role in QS. Furthermore, fish fillets and mutant strains were treated with resveratrol, and the results suggested that resveratrol was an ideal QSI for inhibition of DKPs production via the sb1370 gene and reduced QS in S. baltica, thus delaying the process of fish spoilage during chilling storage.

Journal of Agricultural and Food Chemistry published new progress about Diketopiperazines Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, HPLC of Formula: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Wang, Yanbo; Wang, Feifei; Bao, Xingyue; Feng, Jie; Fu, Linglin published the artcile< Inhibition of biogenic amines in Shewanella baltica by anthocyanins involving a quorum sensing system>, Product Details of C11H18N2O2, the main research area is anthocyanin antibacterial agent biogenic amine quorum sensing Shewanalla; Anthocyanins; Biogenic amines; Diketopiperazines; Quorum sensing inhibitor.

Quorum sensing (QS) is a cell d.-dependent signaling system responsible for various physiol. activities in bacteria. We initially investigated the relation between a QS system and biogenic amine (BA) production in Shewanella baltica, the specific spoilage organism of refrigerated large yellow croaker (Pseudosciaene crocea). In addition, the inhibition effects of anthocyanins from blueberry and purple sweet potato against QS signals and putrescine production were explored. Two kinds of diketopiperazines, cyclo-(l-Pro-l-Leu) and cyclo-(l-Pro-l-Pro), and two kinds of BAs, putrescine and cadaverine, were detected in the culture extract of S. baltica cultivated in sterile large yellow croaker juice, wherein putrescine presented significantly pos. correlations with cyclo-(l-Pro-l-Leu) and cyclo-(l-Pro-l-Pro). In addition, anthocyanins at subminimum inhibitory concentration inhibited the production of diketopiperazines and putrescine in S. baltica 23, a strain with strong putrescine production Furthermore, a transcriptional anal. showed that anthocyanins suppressed the expression of the odc gene in S. baltica, the gene responsible for the production of putrescine from decarboxylation of ornithine. These results established a correlation of the main BA putrescine with the QS system in S. baltica and revealed that anthocyanins could be developed as new QS inhibitors and seafood preservative candidates.

Journal of Food Protection published new progress about Anthocyanins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Product Details of C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Khan, Mohammad Sayyar; Gao, Junlian; Munir, Iqbal; Zhang, Mingfang; Liu, Yixin; Moe, The Su; Xue, Jing; Zhang, Xiuhai published the artcile< Characterization of endophytic fungi, Acremonium sp., from Lilium davidii and analysis of its antifungal and plant growth-promoting effects>, Product Details of C11H18N2O2, the main research area is Acremonium Botrytis Lilium plant growth antifungal agent.

The present study was aimed at isolating endophytic fungi from the Asian culinary and medicinal plant Lilium davidii and analyzing its antifungal and plant growth-promoting effects. In this study, the fungal endophyte Acremonium sp. Ld-03 was isolated from the bulbs of L. davidii and identified through morphol. and mol. anal. The mol. and morphol. anal. confirmed the endophytic fungal strain as Acremonium sp. Ld-03. Antifungal effects of Ld-03 were observed against Fusarium oxysporum, Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi. The highest growth inhibition, i.e., 78.39 ± 4.21%, was observed for B. dothidea followed by 56.68 ± 4.38%, 43.62 ± 3.81%, and 20.12 ± 2.45% for B. cinerea, F. fujikuroi, and F. oxysporum, resp. Anal. of the Et acetate fraction through UHPLC-LTQ-IT-MS/MS revealed putative secondary metabolites which included xanthurenic acid, valyl aspartic acid, gancidin W, peptides, and cyclic dipeptides such as valylarginine, cyclo-[L-(4-hydroxy-Pro)-L-leu], cyclo(Pro-Phe), and (3S,6S)-3-benzyl-6-(4-hydroxybenzyl)piperazine-2,5-dione. Other metabolites included (S)-3-(4-hydroxyphenyl)-2-((S)-pyrrolidine-2-carboxamido)propanoic acid, di-Bu phthalate (DBP), 9-octadecenamide, D-erythro-C18-Sphingosine, N-palmitoyl sphinganine, and hydroxypalmitoyl sphinganine. The strain Ld-03 showed indole acetic acid (IAA) production with or without the application of exogenous tryptophan. The IAA ranged from 53.12 ± 3.20 μg ml-1 to 167.71 ± 7.12 μg ml-1 under different tryptophan concentrations The strain was able to produce siderophore, and its production was significantly decreased with increasing Fe(III) citrate concentrations in the medium. The endophytic fungal strain also showed production of organic acids and phosphate solubilization activity. Plant growth-promoting effects of the strain were evaluated on in vitro seedling growth of Allium tuberosum. Application of 40% culture dilution resulted in a significant increase in root and shoot length, i.e., 24.03 ± 2.71 mm and 37.27 ± 1.86 mm, resp., compared to nontreated control plants. The fungal endophyte Ld-03 demonstrated the potential of conferring disease resistance and plant growth promotion. Therefore, we conclude that the isolated Acremonium sp. Ld-03 should be further investigated before utilization as a biocontrol agent and plant growth stimulator.

BioMed Research International published new progress about Aconitum tuberosum. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Product Details of C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Sun, Xiaowen; Zhang, Run; Ding, Meijuan; Liu, Yongxuan; Li, Lin published the artcile< Biocontrol of the root-knot nematode Meloidogyne incognita by a nematicidal bacterium Pseudomonas simiae MB751 with cyclic dipeptide>, SDS of cas: 2873-36-1, the main research area is cyclic dipeptide biocontrol root Pseudomonas Meloidogyne infection; Meloidogyne incognita; Pseudomonas simiae; cyclic dipeptide; nematicidal activity; root-knot nematode.

Root-knot nematodes (RKNs) are harmful plant-parasitic nematodes that cause serious damage to plant hosts. In the long-term practice of RKN management, bacterial nematicides have attracted increasing attention as an effective biocontrol means. Here we determined the active substances against Meloidogyne incognita from a nematicidal bacterium, developed a biocontrol agent (BCA) based on optimized culture processes. The effects of the BCA on RKN control and plant growth-promotion were evaluated in tomato pot trials. Pseudomonas simiae strain MB751 exhibiting significant nematicidal activity against M. incognita second-stage juveniles (J2) with approx. 80% mortality (with culture supernatant, 96% volume percentage) was isolated from a vineyard. A set of purification and identification experiments was performed to determine the main nematicidal component in MB751. A cyclic dipeptide Cyclo(L-Pro-L-Leu) was identified with a lethal concentration necessary to kill 50% of the population (LC50) of 65.3μg mL-1 against M. incognita J2. Following optimization trials on culture medium/fermentation conditions, such as the single factor test, Plackett-Burman test, steepest ascent, and response surface methodol. experiments, the MB751 fermentation broth was then prepared as a BCA via a cold-air drying process. The BCA and was evaluated in tomato pot experiments for effectiveness in suppressing M. incognita. Significant effects on M. incognita suppression and plant-growth promotion as well as induced systemic resistance to M. incognita of tomato, were observed The cyclic dipeptide-producing bacterium P. simiae MB751 exhibited high nematicidal activity and performance. Further development of this BCA should be pursued for the management of M. incognita in agriculture.

Pest Management Science published new progress about Biological pest control. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Xiang, Wen-Xin; Liu, Qing; Li, Xiao-Man; Lu, Chun-Hua; Shen, Yue-Mao published the artcile< Four pairs of proline-containing cyclic dipeptides from Nocardiopsis sp. HT88, an endophytic bacterium of Mallotus nudiflorus L>, Application of C11H18N2O2, the main research area is cyclic dipeptide isolation structure activity Nocardiopsis; Mallotus nudiflorus (L.) Kulju & Welzen; Nocardiopsis sp. HT88; dd-diketopiperazines; proline (or hydroxyproline)-containing cyclic dipeptides.

Strain HT88 was isolated from the fresh stems of Mallotus nudiflorus, and it was identified as Nocardiopsis sp. by analyzing its morphol. and the 16S rRNA sequence. The extracts of fermented HT88 showed potent antimicrobial activities. Bioassay guided separation of extracts led to 8 proline (or hydroxyproline, Hyp)-containing cyclic dipeptides. Their structures were determined by 1D and 2D NMR spectroscopy and ESI mass spectrometry and further comparison with existing 1H and 13C NMR, m.ps. and sp. rotation data. The 8 2,5-diketopiperazines (DKPs) were identified as cyclo(L-Pro-L-Leu), cyclo(Pro-Leu), cyclo(L-trans-Hyp-L-Leu), cyclo(D-trans-Hyp-D-Leu), and cyclo(D-Pro-L-Phe), cyclo(L-Pro-L-Phe) and cyclo(D-cis-Hyp-L-Phe), cyclo(L-trans-Hyp-L-Phe). Up to date, this is the 1st isolation of 4 pairs of proline-based DKPs from Nocardiopsis sp.

Natural Product Research published new progress about Antibacterial agents. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Application of C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Georgousaki, Katerina; Tsafantakis, Nikolaos; Gumeni, Sentiljana; Gonzalez, Ignacio; MacKenzie, Thomas Andrew; Reyes, Fernando; Lambert, Carole; Trougakos, Ioannis P.; Genilloud, Olga; Fokialakis, Nikolas published the artcile< Screening for tyrosinase inhibitors from actinomycetes; identification of trichostatin derivatives from Streptomyces sp. CA-129531 and scale up production in bioreactor>, Formula: C11H18N2O2, the main research area is Streptomyces trichostatin derivative tyrosinase inhibitors fermentation; Bioreactor; Enzyme kinetics studies; Secondary metabolites, Streptomyces sp.; Trichostatin derivatives; Tyrosinase inhibitors.

In the course of a primary screening of 614 microbial actinomycete extracts for the discovery of tyrosinase inhibitors, the EtOAc extract of the fermentation broth of the strain Streptomyces sp. CA-129531 isolated from a Martinique sample, exhibited in cell free and cell-based assays the most promising activity (IC50 value of 63 μg/mL). Scaled-up production in a bioreactor led to the isolation of one new trichostatic acid analog, namely trichostatic acid B (1), along with six known trichostatin derivatives (2-7), four diketopiperazines (8-11), two butyrolactones (12-13) and one hydroxamic acid siderophore (14). Among them, trichostatin A (4) showed a Ki value of 6.1 μM and six times stronger anti-tyrosinase activity (IC50 2.18 μΜ) than kojic acid (IC50 14.07 μΜ) used as a pos. control. Deoxytrichostatin A (6) displayed also strong inhibitory activity against tyrosinase (IC50 19.18 μΜ). Trichostatin A production in bioreactor started together with the exponential phase of growth (day 4) and the maximum concentration was reached at day 9 (2.67 ± 0.13 μg/mL). Despite the cytotoxicity of some individual components, the EtOAc extract showed no cytotoxic effect on HepG2, A2058, A549, MCF-7 and MIA PaCa-2 cell lines, (IC50 >2.84 mg/mL) and against BG fibroblasts at the concentrations where the whitening effect was exerted, reassuring its safety and great tyrosinase inhibitory potential.

Bioorganic & Medicinal Chemistry Letters published new progress about Fermentation. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Formula: C11H18N2O2.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem

Gonzalez-Dominguez, Raul; Urpi-Sarda, Mireia; Jauregui, Olga; Needs, Paul W.; Kroon, Paul A.; Andres-Lacueva, Cristina published the artcile< Quantitative Dietary Fingerprinting (QDF)-A Novel Tool for Comprehensive Dietary Assessment Based on Urinary Nutrimetabolomics>, Recommanded Product: (3S,8aS)-3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione, the main research area is quant dietary fingerprinting urine nutrimetabolomics; dietary assessment; quantitative dietary fingerprinting (QDF); targeted metabolomics; ultra-high-performance liquid chromatography−tandem mass spectrometry; urine.

Accurate dietary assessment is a challenge in nutritional research, needing powerful and robust tools for reliable measurement of food intake biomarkers. In this work, we have developed a novel quant. dietary fingerprinting (QDF) approach, which enables for the first time the simultaneous quantitation of about 350 urinary food-derived metabolites, including (poly)phenolic aglycons, phase II metabolites, and microbial-transformed compounds, as well as other compounds (e.g., glucosinolates, amino acid derivatives, methylxanthines, alkaloids, and markers of alc. and tobacco consumption). This method was fully validated for 220 metabolites, yielding good linearity, high sensitivity and precision, accurate recovery rates, and negligible matrix effects. Furthermore, 127 addnl. phase II metabolites were also included in this method after identification in urines collected from acute dietary interventions with various foods. Thus, this metabolomic approach represents one-step further toward precision nutrition and the objective of improving the accurateness and comprehensiveness in the assessment of dietary patterns and lifestyles.

Journal of Agricultural and Food Chemistry published new progress about Apple. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Recommanded Product: (3S,8aS)-3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione.

Referemce:
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem