Nishihara, Ryo; Paulmurugan, Ramasamy; Nakajima, Takahiro; Yamamoto, Eiji; Natarajan, Arutselvan; Afjei, Rayhaneh; Hiruta, Yuki; Iwasawa, Naoko; Nishiyama, Shigeru; Citterio, Daniel; Sato, Moritoshi; Kim, Sung Bae; Suzuki, Koji published an article in 2019, the title of the article was Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular events in vivo.Category: pyrazines And the article contains the following content:
Bioluminescence imaging (BLI) is one of the most widely used optical platforms in mol. imaging, but it suffers from severe tissue attenuation and autoluminescence in vivo. Here, we developed a novel BLI platform on the basis of bioluminescence resonance energy transfer (BRET) for achieving a ∼300 nm blue-to-near IR shift of the emission (NIR-BRET) by synthesizing an array of 18 novel coelenterazine (CTZ) derivatives, named “Bottle Blue (BBlue)” and a unique iRFP-linked RLuc8.6-535SG fusion protein as a probe. The best NIR-BRET was achieved by tuning the emission peaks of the CTZ derivatives to a Soret band of the iRFP. In mammalian cells, BBlue2.3, one of the CTZ derivatives, emits light that is ∼50-fold brighter than DBlueC when combined with RLuc8.6-535SG, which shows stable BL kinetics. When we used a caged version of BBLue2.3, it showed a BL half decay time of over 60 min while maintaining the higher signal sensitivity. This NIR BL is sufficiently brighter to be used for imaging live mammalian cells at single cell level, and also for imaging metastases in deep tissues in live mice without generating considerable autoluminescence. A single-chain probe developed based on this BLI platform allowed us to sensitively image ligand antagonist-specific activation of estrogen receptor in the NIR region. This unique optical platform provides the brightest NIR BLI template that can be used for imaging a diverse group of cellular events in living subjects including protein-protein interactions and cancer metastasis. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).Category: pyrazines
The Article related to nir bioluminescence resonance energy transfer coelenterazine, bioluminescence imaging, bioluminescence resonance energy transfer (bret), blue-to-near infrared shift, coelenterazine derivatives, metastasis and other aspects.Category: pyrazines