Abe, Masahiro; Nishihara, Ryo; Kim, Sung-Bae; Suzuki, Koji published an article in 2021, the title of the article was Near-infrared bioluminescence imaging of animal cells with through-bond energy transfer cassette.HPLC of Formula: 55779-48-1 And the article contains the following content:
A review. Coelenterazine (CTZ) is the most general substrate for marine luciferases. The present protocol introduces a near-IR (NIR) bioluminescence (BL) imaging of mammalian cells with a cyanine-5 (Cy5) dye-conjugated CTZ. This unique Cy5-conjugated CTZ, named Cy5-CTZ, can act as a dual optical readout emitting both fluorescence (FL) and BL. The Cy5-CTZ exerts through-bond energy transfer (TBET)-based imaging modalities for mammalian cells. This novel derivative, Cy5-CTZ, is intrinsically fluorescent and emits NIR-shifted BL when reacting with an appropriate luciferase, such as Renilla luciferase (RLuc). The protocol exemplifies a unique live-cell imaging with Cy5-CTZ that is optically stable in physiol. samples and rapidly permeabilize through plasma membrane and emit NIR-BL in live mammalian cells. The experimental process involved the reaction of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one(cas: 55779-48-1).HPLC of Formula: 55779-48-1
The Article related to review energy transfer cassette bioluminescence imaging, bioluminescence (bl), coelenterazine (ctz), cyanine-5 (cy5), near infrared (nir), through-bond energy transfer (tbet) and other aspects.HPLC of Formula: 55779-48-1