Yang, Bo; Yang, Zhong-Duo; Li, Xiao-Fei; Shu, Zong-Mei published the artcile< Secondary Metabolites of the Endophytic Fungi Talaromyces wortmannii Cultivated in Maize Medium and their Bioactivity>, Synthetic Route of 2873-36-1, the main research area is Talaromyces sitosterol deacetylisowortmin aspergillumarin.
In this paper, we described the isolation and identification of eight compounds (1-8) from an endophytic fungus LGT-4 (identified as Talaromyces wortmannii) of Tripterygium wilfordii. The fungal strain (LGT-4) was isolated from the roots of Tripterygium wilfordii and identified as Talaromyces wortmannii based on both morphol. on PDA and anal. of the DNA sequences of the ITS1-5.8S-ITS2 rRNA gene region. A GenBank search for DNA sequence (GenBank Accession Number KF850714) similarity revealed that ITS1-5.8S-ITS2 of LGT-4 was 99% homologous to that of Talaromyces wortmannii reference strains (GenBank Accession Numbers FR667650.1 and JN807336.1). The strain Lgt-4 was cultivated in maize medium (100 mL × 80 Erlenmeyer flask) for 40 days at 28°C in a constant temperature oscillation incubator. The culture was extracted with EtOAc (8 L) to afford the crude extract (26 g), which was subjected to column chromatog. using silica gel, Sephadex LH-20, MCI-CHP20P gel, and HPLC, etc. A total of eight compounds was isolated and identified based on NMR spectra. To the best of our knowledge, this is the first report on the isolation of the 4, 5, and 7 from Talaromyces wortmannii
Chemistry of Natural Compounds published new progress about Bioactive amplification with probing assay. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.