Senturk, Melih; Ercan, Fahriye; Yalcin, Serap published the artcile< The secondary metabolites produced by Lactobacillus plantarum downregulate BCL-2 and BUFFY genes on breast cancer cell line and model organism Drosophila melanogaster: molecular docking approach>, Synthetic Route of 2873-36-1, the main research area is Lactobacillus Drosophila breast cancer secondary metabolite mol docking; Apoptosis; Drosophila melanogaster; Gene expression; Lactobacillus plantarum; MCF-7; Molecular docking.
The current study was designed to evaluate the toxicity of the secondary metabolites of Lactobacillus plantarum against the human breast cancer cell line (MCF-7) and the Drosophila melanogaster. In this study, toxicity analyses of secondary metabolites of Lactobacillus plantarum were analyzed on breast cancer cells, and the Drosophila melanogaster. After application, in the MCF-7 cell line, expression levels of RRAS-2, TP53, BCL-2, APAF-1, CASP-3, FADD, CASP-7, BOK genes; in D. melanogaster; expression levels of RAS64B P53, BUFFY, DARK, DECAY, FADD, DRICE, and DEBCL genes were determined by RT-PCR. In addition, anal. of L. plantarum secondary metabolite was performed by GC-MS method and mol. binding poses of secondary metabolites and human enzymes were investigated in silico. Drosophila melanogaster being used as a model organism where some of the human genes were preserved. The IC50 value of the secondary metabolite in the MCF-7 cell line was determined to be 0.0011 mg/mL. Lethal concentration 50 (LC50) and 99 (LC99) values of secondary metabolites against fruit fly adults were 0.24 mg/mL and 0.54 mg/mL, resp. The substance detected in the secondary metabolite content and encoded as L13 (3-phenyl-1, 2, 4-benzotriazine) has been observed to have high binding affinity in the studied genes.
Cancer Chemotherapy and Pharmacology published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (DARK). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.