Chauhan, Priyanka; Bhattacharya, Arpita; Giri, Ved Prakash; Singh, Satyendra Pratap; Gupta, Sateesh Chandra; Verma, Pratibha; Dwivedi, Ashish; Rajput, Laxman Singh; Mishra, Aradhana published the artcile< Bacillus subtilis suppresses the charcoal rot disease by inducing defence responses and physiological attributes in soybean>, Synthetic Route of 2873-36-1, the main research area is Bacillus subtilis soybean charcoal rot disease physiol; Bacillus subtilis; Charcoal rot disease; GC–MS profiles; Macrophomina phaseolina; Secondary metabolites; Soybean.
Endophytes can induce the defense responses and modulates physiol. attributes in host plants during pathogen attacks. In the present study, 127 bacterial endophytes (BEs) were isolated from different parts of healthy soybean plant. Among them, two BEs (M-2 and M-4) resulted a significant antagonistic property against Macrophomina phaseolina, causes charcoal rot disease in soybean. The antagonistic potential was evaluated through dual culture plate assay, where M-4 expressed higher antifungal activity than M-2 against M. phaseolina. The M-4 produces cell wall degrading enzymes viz. cellulase (145.71 ± 1.34 μgmL-1), chitinase (0.168 ± 0.0009 unitmL-1) and β,1-3 endoglucanase (162.14 ± 2.5 μgmL-1), which helps in cell wall disintegration of pathogens. Addnl., M-4 also can produce siderophores, indole-3-acetic acid (IAA) (17.03 ± 1.10 μgmL-1) and had a phosphate solubilization potential (19.89 ± 0.26 μgmL-1). Further, GC-MS profiling of M-4 has been carried out to demonstrate the production of lipophilic secondary metabolites which efficiently suppress the M. phaseolina defensive compounds under co-culture conditions. Bio-efficacy study of M-4 strain shown a significant reduction in disease incidence around 60 and 80% in resistant and susceptible varieties of soybean, resp. The inoculation of M-4 potentially enhances the physiol. attributes and triggers various defense responsive enzymes viz. superoxide dismutase (SOD), phenol peroxidase (PPO), peroxidase (PO) and catalase (CAT). The histopathol. study also confirmed that M-4 can reduce the persistence of microsclerotia in root and shoot tissue. Conclusively, M-4 revealed as an efficient biocontrol agent that can uses multifaceted measures for charcoal rot disease management, by suppress the M. phaseolina infection and enhance the physiol. attributes of soybean.
Archives of Microbiology published new progress about Bacillus subtilis. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Synthetic Route of 2873-36-1.