Salman, Mahwish; Tariq, Anam; Mustafa, Ghulam; Javed, Muhammad Rizwan; Naheed, Shazia; Qamar, Sarmad Ahmad published the artcile< Cyclo(L-Leucyl-L-Prolyl) from Lactobacillus coryniformis BCH-4 inhibits the proliferation of Aspergillus flavus: an in vitro to in silico approach>, Reference of 2873-36-1, the main research area is Lactobacillus Aspergillus proliferation Cyclo L Leucyl Prolyl antifungal bioprotectant; Antifungal potential; Aspergillus flavus; Bioprotectant; Cyclo(L-Leu-L-Pro); Lactobacillus coryniformis; Molecular docking.
Fungal spoilage led to a considerable economic loss of foodstuff which ultimately affects public health due to mycotoxins production Moreover, the consumption of com. antifungal drugs creates side effects and develops antifungal resistance. To overcome these challenges, the current work was aimed to investigate novel antifungal cyclic dipeptide (CDP) from Lactobacillus coryniformis (Loigolactobacillus coryniformis) BCH-4. CDPs have flexible, cyclic, and stable conformation. The proline-based CDPs provide addnl. structural compatibility and bio-functional values. Keeping in view, high-performance liquid chromatog. (HPLC) was performed to explore cyclo(L-Leu-L-Pro) from L. coryniformis BCH-4. The HPLC detected concentration (135 ± 7.07 mg/mL) exhibited in vitro antifungal activity of 5.66 ± 0.57 mm (inhibitory zone) against Aspergillus flavus. Based on these results, cyclo(L-Leu-L-Pro) was used as a bioprotectant for selected food samples (grapes, lemon, cashew nuts, and almonds). A significant impact of cyclo(L-Leu-L-Pro) was observed in contrast with MRS broth (control) and cell-free supernatant. In silico mol. docking anal. of this CDP was carried out against FAD glucose dehydrogenase, dihydrofolate reductase, and urate oxidase of A. flavus as target proteins. Among these proteins, FAD glucose dehydrogenase exerted strong interactions with cyclo(L-Leu-L-Pro) having S-score of – 8.21. The results evaluated that the detected CDP has strong interactions with selected proteins, causing excellent growth inhibition of A. flavus. Therefore, cyclo(L-Leu-L-Pro) could be used as a potent bioprotectant against food-borne pathogenic fungi.
Archives of Microbiology published new progress about Almond. 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Reference of 2873-36-1.