Floris, Patrick; McGillicuddy, Nicola; Morrissey, Brian; Albrecht, Simone; Kaisermayer, Christian; Hawe, David; Riordan, Lelia; Lindeberg, Anna; Forestell, Sean; Bones, Jonathan published the artcile< A LC-MS/MS platform for the identification of productivity markers in industrial mammalian cell culture media>, Application In Synthesis of 2873-36-1, the main research area is mammalian cell CHO cell.
Cell culture media formulations supplemented with soy hydrolyzates for com. scale manufacturing of therapeutic proteins were characterized by high-resolution LC-MS/MS to identify specific media components that contributed to cellular bioprocess variability. Initial untargeted screening of media formulations resulted in the combined quantification of >2500 features upon anal. by reversed-phase and hydrophilic interaction liquid chromatog. Chemometric evaluation of quantified features by Principal Component Anal. and Hierarchical Clustering revealed clear correlation between media lots and the corresponding hydrolyzates implemented during formulation development stages, suggesting that the observed variation in culture performance could be influenced by components present within hydrolyzates. Through multivariate data anal., a small number of dipeptides, identified as Tyrosyl-Leucine, Phenylalanyl-Valine and LL-Cyclo(Leucylprolyl) were determined in media as potential contributors to bioprocess variability due to their statistically significant higher abundance in formulations which yielded low cellular productivity. Subsequent quantification of these features was performed through targeted LC-QQQ-MS/MS which however, revealed discrepancies between dipeptide concentration levels in media and corresponding hydrolyzates presumably due to their potential instability upon interaction with other chem. defined components such as metals or chelating agents. The nutritional impact of the aforementioned features of interest on CHO cell growth performance was assessed through spiking studies in representative shake-flask cultures which however, failed to demonstrate clear correlation between dipeptide concentrations and achieved product yields further indicating the presence of a plausible synergetic effect between the identified dipeptides and alternative media components.
Process Biochemistry (Oxford, United Kingdom) published new progress about Growth (mammalian cell). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, Application In Synthesis of 2873-36-1.