Begum Ahil, Sajeli; Hira, Kirti; Shaik, Ameer Basha; Pal, Pragya Paramita; Kulkarni, Onkar Prakash; Araya, Hiroshi; Fujimoto, Yoshinori published the artcile< L-Proline-based-cyclic dipeptides from Pseudomonas sp. (ABS-36) inhibit pro-inflammatory cytokines and alleviate crystal-induced renal injury in mice>, SDS of cas: 2873-36-1, the main research area is sequence Pseudomonas proline cyclic dipeptide renal injury proinflammatory cytokine; Cyclic dipeptides; Cyclo(Val-Pro); IL-1β; IL-6; Oxalate-induced nephropathy; Pseudomonas; Renal inflammation; TNF-α.
Study on the constituents of bioactive culture broth extract (CBE) of Pseudomonas sp. (ABS-36) explored the secretion of an array of cyclic dipeptides (CDPs) and twenty of them had been isolated and reported in the present paper. Six major CDPs [cyclo(Leu-Pro) (1), cyclo(Val-Pro) (2), cyclo(Leu-hydroxy-Pro) (9), cyclo(Pro-Tyr) (10), cyclo(Pro-Ala) (11) and cyclo(Gly-Pro) (12)] exhibited pan cytokine inhibition effect by inhibiting key pro-inflammatory cytokines IL-1β, TNF-α and IL-6 tested under various cell based assays. With this background, the effect of these six CDPs in treating renal inflammation was screened using crystal-induced nephropathy model in mice at 50 mg/kg body weight through oral administration. cis-Cyclo(Val-Pro) (2) exhibited 57% inhibition of plasma IL-1β protein expression and 35.2% inhibition of elevated blood urea nitrogen. Further, cis-cyclo(Val-Pro) (2) attenuated renal injury as demonstrated by significant reduction of mRNA expressions of IL-1β (P < 0.01) and kidney injury marker-1 (P < 0.001). Furthermore, evaluation of tubular-necrosis, -dilation and -cast in the histol. sections exhibited moderate protection of renal tissues by cis-cyclo(Val-Pro) (2). All the tested CDPs reduced the nitrite production and were interestingly non-cytotoxic. The isolated fluorescing bacterial strain was identified as Pseudomonas species by comparison of 16S rDNA sequence and registered as ABS-36 strain GenBank Accession Number KT625586. International Immunopharmacology published new progress about 16S rRNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2873-36-1 belongs to class pyrazines, and the molecular formula is C11H18N2O2, SDS of cas: 2873-36-1.