The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid( cas:2150-55-2 ) is researched.Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid.Yang, Bo; Liu, Zhigang; Deng, Bei; Zeng, Yulei; Hu, Jiajun; Li, Weilin; Hu, Zheng published the article 《Isolation and genetic improvement of Pseudomonas sp. strain HUT-78, capable of enzymatic production of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid》 about this compound( cas:2150-55-2 ) in Journal of General and Applied Microbiology. Keywords: cysteine production Pseudomonas fermentation mutagenesis genetic engineering; aminothiazolinecarboxylate hydrolase carbamoylcysteine amidohydrolase Pseudomonas. Let’s learn more about this compound (cas:2150-55-2).
Microorganisms able to bioconvert DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) into L-cysteine were originally isolated from 10 soil samples with DL-ATC as the sole nitrogen source. Ninety-seven L-cysteine-producing bacterial strains were screened out and obtained in pure culture. Among them, a strain, designated as HUT-78, was selected as the best producer, with a molar bioconversion rate of 60%. Based on the 16S rRNA gene sequence anal., this isolate was placed within the genus Pseudomonas. A novel mutant of this strain with a significantly reduced activity of L-cysteine desulfhydrase, a L-cysteine-decomposing enzyme, was derived by UV-mutagenesis. This mutant, designated as mHUT-78, exhibited a 42% increase in L-cysteine producing activity. Moreover, the bioconversion reactions in both the parent and the mutant strain were significantly accelerated by co-overexpression of the two key enzymes, AtcB and AtcC, involved in the bioconversion reaction.
Although many compounds look similar to this compound(2150-55-2)Name: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, numerous studies have shown that this compound(SMILES:O=C(C1N=C(N)SC1)O), has unique advantages. If you want to know more about similar compounds, you can read my other articles.