Most of the compounds have physiologically active properties, and their biological properties are often attributed to the heteroatoms contained in their molecules, and most of these heteroatoms also appear in cyclic structures. A Journal, Tianjin Keji Daxue Xuebao called Optimization of fermentation conditions of microbial enzymatic synthesis of L-cysteine, Author is Kou, Guang-hui; Huai, Li-hua; Bai, Gang; Yang, Wen-bo, which mentions a compound: 2150-55-2, SMILESS is O=C(C1N=C(N)SC1)O, Molecular C4H6N2O2S, Formula: C4H6N2O2S.
Pseudomonas sp. TS1138 was used as the test strain to produce L-cysteine, and microbial enzymic synthesis of L-cysteine was studied. In the medium optimization, it was found that glucose and urea were the best carbon and nitrogen source for enzyme production, resp. Effect of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) on enzyme production was carried out, and the exptl. result showed that a initial concentration of 5 g/L DL-ATC was found to be optimal for enzyme production Effects of inoculum size, initial pH and liquid volume on enzyme production were investigated. The results indicated that the optimum inoculum size was 10%, and the optimum initial pH was 8.0. The optimum liquid volume was 40 mL culture medium in a 500 mL shaking flask.
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