Category: pyrazines. The mechanism of aromatic electrophilic substitution of aromatic heterocycles is consistent with that of benzene. Compound: 2-Amino-4,5-dihydrothiazole-4-carboxylic acid, is researched, Molecular C4H6N2O2S, CAS is 2150-55-2, about Optimization of bioconversion conditions for manufacturing L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid with immobilized cells based on response surface analysis. Author is Wang, Pu; Wang, Hao; Zhang, Shi-jie; Zhang, Xiao-jun.
The conversion conditions for manufacturing L-cysteine using immobilized cells were optimized by using SAS software combined with the methods of Plackett-Burman design and response surface methodol. The cells were immobilized by calcium alginate embedding method. The optimum levels of three important factors were determined as follows: the volume of immobilized cell was 25.5 mL, the concentration of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) was 1.0 mass%, and the proliferation time of immobilized cells was 12.9 h. Experiments showed that the average enzyme activity could reach 934 U/mL at optimized conditions for five batches, with an increase of 38.9% compared with that before the optimization. After the immobilized cells were utilized for 4 times, the conversion rate could be still over 91.0% of the initial value.
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