Researchers who often do experiments know that organic synthesis is a process of preparing more complex target molecules from simple raw materials through one or more chemical reactions. Generally, it requires fewer steps, and cheap raw materials. 21279-64-1, name is 5-Chloropyrazine-2-carboxamide, A new synthetic method of this compound is introduced below., HPLC of Formula: C5H4ClN3O
5-Chloropyrazinamide (1, 50 mg, 0.3173 mmol), potassiumfluoride (KF) (110.43 mg, 1.90 mmol) and tetra-n-butylammonium bromide (TBABr) (20.46mg, 0.064 mmol) were added to a 10 mL RBF and placed under vacuum for 1 hour to dry.After filling the RBF with N2, 10 mL of dry DMSO stored over molecular sieves was added tothe RBF. The reaction mixture was heated to 110 C and refluxed for 30 min under N2. Afterthe reaction was shown to be complete by TLC, the mixture was cooled to room temperature, diluted with iced water and then extracted with ethyl acetate. The organic layer was dried withMgSO4 and the solvent was evaporated by vacuum to yield crude product which was purifiedby CombiFlash chromatography using a silica column and petroleum ether and ethyl acetateas the eluent.GC-EI Mass. Calculated for C5H4FN3O (M·+) 141.11, found 141.11. Two fragments peakswere also observed: M-CONH2·+ (C4H2FN2·+), m/z = 97.07 and M-CONH+ (C4H2FN2H+),m/z = 98.08.1H NMR (400 MHz, CDCl3 and CD3OD): delta 9.00 (s, 1H), delta 8.36 (d, J = 7.8 Hz, 1H). 19FNMR (400 MHz, CDCl3 and CD3OD): delta 75.17 (d, J = 7.3 Hz). 13C NMR (500 MHz, CDCl3and CD3OD): delta 164.72 (s, 1′-C), delta 161.56 (d, J = 256.5 Hz, 5′-C), delta 142.41 (d, J = 12.8 Hz, 3′-C),delta 142.27 (d, 2′-C) delta 131.78 (d, J = 38.7 Hz, 6′-C)
The synthetic route of 21279-64-1 has been constantly updated, and we look forward to future research findings.
Reference:
Article; Zhang, Zhuo; Ordonez, Alvaro A.; Smith-Jones, Peter; Wang, Hui; Gogarty, Kayla R.; Daryaee, Fereidoon; Bambarger, Lauren E.; Chang, Yong S.; Jain, Sanjay K.; Tonge, Peter J.; PLoS ONE; vol. 12; 2; (2017);,
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem