In the chemical reaction process, reaction time, type of solvent, can easily affect the result of the reaction, thereby determining the yield and properties of the reaction product. An updated downstream synthesis route of 59489-32-6 as follows. Computed Properties of C6H7ClN2
Pd2(dba)3-CHC13 (5 mg, 0.5 mol %), ligand 1 (8 mg, 2 mol %) and 1.4 eq sodium tert- butoxide (13 mg, 0.14 mmol) were weighed in air and transferred into a microwave tube, followed by dioxane (750 muL), 1.0 eq JVrlambda/-dimethyl-l-(piperidin-4-yl)-2’/f-spiro[piperidine-4,41- quinolineJ-rp’i^O-carboxamide (36 mg, 0.10 rnmol) and 1.0 eq 5-chloro-2,3-dimethylpyrazine (14 mg). The tube was flushed with nitrogen, capped and stirred at 800C for 3 hours. The reaction was cooled to room temperature, diluted with methanol (500 muL), filtered (Whatman 0.45 mum PTFE) and subjected to reverse-phase HPLC purification (2-40% CH3CN gradient [w/ 0.1 % TFA (aq)] over / = 10 minutes, 750 muL injected, 35 mL/min) to yield compound no. 20. LC/MS (10-99% CH3CN/0.05% TFA gradient over 5 min): m/z 463.4, retention time 2.13 minutes.
According to the analysis of related databases, 59489-32-6, the application of this compound in the production field has become more and more popular.
Reference:
Patent; VERTEX PHARMACEUTICALS INCORPORATED; WO2007/100670; (2007); A1;,
Pyrazine – Wikipedia,
Pyrazine | C4H4N2 – PubChem