Researchers who often do experiments know that organic synthesis is a process of preparing more complex target molecules from simple raw materials through one or more chemical reactions. Generally, it requires fewer steps, and cheap raw materials. 61442-38-4, name is 6-Aminopyrazine-2-carboxylic acid, A new synthetic method of this compound is introduced below., COA of Formula: C5H5N3O2
A mixture of l-(3-((5-aminopyridin-3-yl)amino)-5-methyl-5H-chromeno[4,3- c]pyridin-8-yl)pyrrobdin-2-one (60 mg, 0.14 mmol, HC1 salt), 6-aminopyrazine-2-carboxybc acid (24 mg, 0.17 mmol) and EDCI.HC1 (30 mg, 0.16 mmol) in pyridine (2 mL) was heated at 50 C for 2 h. A black solution was formed. LCMS (Rt = 0.687 min; MS Calcd: 508.2; MS Found: 509.1 [M+H]+). The mixture was concentrated and the residue was poured into water (20 mL) and stirred for 2 minutes. The aqueous layer was extracted with ethyl acetate (20 mL x3). The combined organic layer was washed with water (20 mL x2) and brine (20 mL), dried over anhydrous Na2S04, filtered and concentrated. The residue was purified by prep-HPLC (0.225% FA as an additive) and lyophilized to give 6-amino-N-(5-((5-methyl-8-(2- oxopyrrolidin-l-yl)-5H-chromeno[4,3-c]pyridin-3-yl)amino)pyridin-3-yl)pyrazine-2- carboxamide (23.5 mg, yield: 32%) as a yellow solid. (1570) NMR (400 MHz DMSO-rie) d 1.55 (3H, d, J= 6.5 Hz), 2.01-2.10 (2H, m), 2.52-2.54 (2H, m, overlapped with the peak of DMSO), 3.84 (2H, t, J= 7.7 Hz), 5.30 (1H, q , J= 6.4 Hz), 6.80 (1H, s), 6.82 (2H, brs), 7.33 (1H, dd, J= 8.7, 2.1 Hz), 7.41 (1H, d, J= 2.3 Hz), 7.90 (1H, d, J= 8.8 Hz), 8.15 (1H, s), 8.36 (1H, s), 8.55 (1H, s), 8.70 (1H, s), 8.76-8.83 (2H, m), 9.71 (1H, brs), 10.46 (1H, brs).
The basis of chemical reaction formula synthesis, the synthesis route is composed of some specific reactions and combined according to certain logical thinking. We look forward to the emergence of more reaction modes in the future.